Rekombinanter CD44 Antikörper

Produktnummer ABIN7072358

Der Ratte Monoklonal Anti-CD44-Antikörper wurde für FACS, WB, IHC, ELISA, IF, IP, ICC, Neut und BP validiert. Er ist geeignet, CD44 in Proben von Human, Maus, Ratte, Kaninchen, Rind (Kuh), Schaf, Ziege und Primate zu detektieren.

Kurzübersicht für Rekombinanter CD44 Antikörper (ABIN7072358)

Target: CD44

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Maus, Ratte, Kaninchen, Rind (Kuh), Schaf, Ziege, Primate

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD44 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Neutralization (Neut), Blocking Peptide (BP)

Klon: Hermes-1

Produktdetails anti-CD44 Antikörper

Verwendungszweck: Anti-CD44 [Hermes-1], Rat IgG2a, kappa

Spezifität: This antibody is specific for CD44 (gp90 epitope cluster I), which is selectively expressed on normal or transformed lymphoid cells that are able to recognize and bind to high endothelial venules (specialized vessels that mediate lymphocyte exit from the blood into lymphoid organs). Although this antibody was raised against human CD44, it cross-reacts with murine, rat, rabbit, primate, sheep, bovine and goat CD44.

Kreuzreaktivität: Rind (Kuh), Ziege, Maus, Primate, Kaninchen, Ratte, Schaf

Produktmerkmale:

Original Species of Ab: Rat

Original Format of Ab: IgG2a

Aufreinigung: Protein A affinity purified

Immunogen: This antibody was produced by immunizing a Fischer rat with human tonsil lymphocytes (10^7cells intraperitoneally in Freund's complete adjuvant). A boost was given 2 weeks later (intravascularly in saline).

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: This antibody binds CD44, so can be used to label CD44+ cells in flow cytometry analysis. As CD44 Molecules are used to bind to high endothelial venules (HEV) at the site of lymphoctye entry to lymphoid organs, this antibody can be used to specifically stain HEV-binding cell lines (Jalkanen, 1986). In addition, this antibody blocks the adhesion of the haematopoietic progenitor cell line KG1 to hyaluronic acid (Bendall, 2003), but does not block lymphocyte binding to HEV (Picker, 1989). 7H9D6 partially blocks the binding of this antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu CD44

Target: CD44

Andere Bezeichnung: CD44

Hintergrund: CDw44, Epican, Extracellular matrix receptor III, ECMR-III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, PGP-1, Phagocytic glycoprotein I, CD44/H-CAM antibody, Hermes 1

UniProt: P16070

Pathways: Glycosaminoglycan Metabolic Process, Autophagie, Negative Regulation of intrinsic apoptotic Signaling