Rekombinanter Ly6g Antikörper

Produktnummer ABIN7072246

Dieser Kaninchen Chimeric Antikörper detektiert spezifisch Ly6g in FACS und Dep. Es zeigt Reaktivität gegenüber Proben von Maus.

Kurzübersicht für Rekombinanter Ly6g Antikörper (ABIN7072246)

Target: Ly6g (Lymphocyte Antigen 6 Complex, Locus G (Ly6g))

Antikörpertyp: Recombinant Antibody

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser Ly6g Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Depletion (Dep)

Klon: 1A8

Produktdetails anti-Ly6g Antikörper

Verwendungszweck: Anti-Ly6G [1A8], Rabbit IgG, kappa

Spezifität: Recognises the murine GPI-anchored protein Ly6G, a marker of granulocyte differentiation, maturation and, together with Ly6C, a component of the Granulocyte Receptor-1 Antigen (Gr-1). Unlike another commonly used clone, RB6-8C5, 1A8 does not bind Ly6C.

Produktmerkmale:

Original Species of Ab: Rat

Original Format of Ab: IgG2a

Aufreinigung: Protein A affinity purified

Immunogen: LEW/N rats immunized with 1.5x10E8 Ly-6G-transfected EL4J cells

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: Neutrophils are crucial components of our immune system. Ly6G is highly expressed on neutrophils, which allows the 1A8 clone to be used not only in detection of neutrophils, but also in selective depletion < a href="http://www.ncbi.nlm.nih.gov/pubmed/17884993" target="_blank"> in vivo. It may also be used as a negative selection marker for bone-marrow derived macrophages, which should be Ly6G(-) and F4/80 (+) < a href="http://absoluteantibody.com/ab/anti-f480-cia3-1-recombinant-version/" target="_blank"> (see our F4/80 range of antibodies) . The mouse IgG2a version of the antibody, recombinantly produced by Absolute Antibody, could deplete neutrophils in mice more efficiently than the rat version of the antibody. Further, this version increased depleting effector function, and prevented the production of anti-rat antibodies (Olofsen et al., 2022, PMID: 36359801).

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu Ly6g

Target: Ly6g (Lymphocyte Antigen 6 Complex, Locus G (Ly6g))

Andere Bezeichnung: Ly6G

Hintergrund: LGR-1 , y-6G, GR1, Lymphocyte antigen 6G

UniProt: P35461