TRPV1 Antikörper (C616S, Extracellular)

Produktnummer ABIN7043838

Dieses Anti-TRPV1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von TRPV1 in WB, IHC, IF, FACS, LCI und IC. Geeignet für Ratte.

Kurzübersicht für TRPV1 Antikörper (C616S, Extracellular) (ABIN7043838)

Target: TRPV1 (Transient Receptor Potential Cation Channel, Subfamily V, Member 1 (TRPV1))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TRPV1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Live Cell Imaging (LCI), Immunochromatography (IC)

Produktdetails anti-TRPV1 Antikörper

Bindungsspezifität: AA 605-619, C616S, Extracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to Rat TRPV1 (VR1) Channel

Spezifität: 3rd extracellular loop

Homologie: Rat - 14 out of 15 amino acid residues identical Mouse - 12 out of 15 amino acid residues identicalWon't recognize TRPV1 from human samples

Produktmerkmale: Anti-Rat TRPV1 (VR1) (extracellular) Antibody (ABIN7043838, ABIN7043986 and ABIN7043987) is a highly specific antibody directed against an extracellular epitope of the rat TRPV1 channel. The antibody can be used in western blot, immunohistochemistry, and live cell imaging applications. It has been designed to recognize TRPV1 from rat samples only.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)NSLPMESTPHK*SRGS, corresponding to amino acid residues 605-619 of rat TRPV1 with replacement of cysteine 616 (C616) with serine (*S)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu TRPV1

Target: TRPV1 (Transient Receptor Potential Cation Channel, Subfamily V, Member 1 (TRPV1))

Andere Bezeichnung: TRPV1

Hintergrund: Transient receptor potential cation channel subfamily V member 1, Capsaicin receptor, Vanilloid receptor 1, OTRPC1,TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology, TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family, the TRPA, TRPML, and TRPP).1-4 The TRPV subfamily consists of six members, TRPV1-6.5TRPV1 channel has many activators among them heat, protons, vanilloids like capsaicin, reiniferatoxin (RTX), and lipids. This channel is associated with tissue injury and inflammation.6,7TRPV1 is expressed predominantly in nociceptors and in sensory neurons.Recent studies demonstrated involvement of TRPV1 in apoptosis where inhibition of the receptor prevented apoptosis.8,9

Alternative names: TRPV1 (VR1), Transient receptor potential cation channel subfamily V member 1, Capsaicin receptor, Vanilloid receptor 1, OTRPC1

Gen-ID: 83810

NCBI Accession: NM_018727

UniProt: O35433

Pathways: Dicarboxylic Acid Transport