TRPM4 Antikörper (Intracellular)

Produktnummer ABIN7043833

Produktdetails anti-TRPM4 Antikörper

Target: TRPM4 (Transient Receptor Potential Cation Channel, Subfamily M, Member 4 (TRPM4))

Bindungsspezifität: AA 5-17, Intracellular

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TRPM4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunochromatography (IC), Immunofluorescence (IF)

Verwendungszweck: A Rabbit Polyclonal Antibody To TRPM4 Channel

Spezifität: Intracellular, N-terminus

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Rat,mouse - 12,13 amino acid residues identical

Produktmerkmale: Anti-TRPM4 Antibody is directed against an epitope of human TRPM4. Anti-TRPM4 Antibody (ABIN7043833, ABIN7044008 and ABIN7044009) can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize TRPM4 from human, rat, and mouse samples.

Aufreinigung: Affinity purified on immobilized antigen.

Güteklasse: KO Validated

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: EKEQSWIPKIFKK(C), corresponding to amino acid residues 5-17 of human TRPM4

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Kommentare:

Cited Application: IHC|ICC|IFC

Negative Control: (ABIN7237454)

Blocking Peptide: (ABIN7237454)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu TRPM4

Target: TRPM4 (Transient Receptor Potential Cation Channel, Subfamily M, Member 4 (TRPM4))

Andere Bezeichnung: TRPM4 (TRPM4 Produkte)

Hintergrund: Melastatin-4, LTrpC4, Calcium-activated non-selective cation channel 1,The mammalian melastatin-related transient receptor potential (TRPM) is a subfamily of the TRP family. The family was named after the first discovered member, melastatin (TRPM1) whose gene was identified in metastatic and benign melanomas1-3. The TRPM family consists of eight members designated as TRPM1-8 that can be further divided into four pairs: TRPM1 and TRPM3, TRPM2 and TRPM8, TRPM4 and TRPM5, and TRPM6 and TRPM71,2.The TRPM proteins share structural homology with other members of the TRP superfamily channels, six putative transmembrane domains, and cytoplasmic N- and C-termini. However, due to their long N- and C-termini they are also named the long TRP channel family1-3. TRPM4 and TRPM5 are the only TRP channels that are permeable to monovalent cations but not to Ca2+. TRPM4 is expressed as at least two alternative spliced isoforms, TRPM4a and TRPM4b which consist of shorter and longer N-terminal regions, respectively. TRPM4a displays little activity, TRPM4b and TRPM5 appear to be directly activated by an increase in intracellular Ca2+ concentration4.TRPM4 is expressed at relatively high levels in cardiac tissues.

Alternative names: TRPM4, Melastatin-4, LTrpC4, Calcium-activated non-selective cation channel 1

Gen-ID: 54795

NCBI Accession: NM_017636

UniProt: Q8TD43

Pathways: Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response