SLC7A11 Antikörper (Extracellular) (PE)

Produktnummer ABIN7043753

Der Kaninchen Polyklonal anti-SLC7A11 Antikörper wird verwendet zum Nachweis von SLC7A11 in Proben von Maus. Er wurde validiert für FACS.

Kurzübersicht für SLC7A11 Antikörper (Extracellular) (PE) (ABIN7043753)

Target: SLC7A11 (Solute Carrier Family 7, (Cationic Amino Acid Transporter, Y+ System) Member 11 (SLC7A11))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SLC7A11 Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS)

Produktdetails anti-SLC7A11 Antikörper (PE)

Bindungsspezifität: AA 218-231, Extracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to xCT/SLC7A11 Conjugated to the Fluorescent Dye R-Phycoerythrin

Spezifität: 3rd extracellular loop

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Rat - identical, human - 11,14 amino acid residues identical

Produktmerkmale: Anti-xCT/SLC7A11 (extracellular) Antibody (ABIN7043751, ABIN7044835 and ABIN7044836) is a highly selective antibody directed against an epitope of mouse cystine/glutamate transporter. The antibody can be used in western blot analysis. It has been designed to recognize xCT from human, mouse, and rat samples. \nAnti-xCT/SLC7A11 (extracellular)-PE Antibody (ABIN7043753, ABIN7045636, ABIN7045637, ABIN7045638 and ABIN7045639) is directly conjugated to Phycoerythrin (PE). This labeled antibody can be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)THHFKDAFSGRDTS, corresponding to amino acid residues 218-231 of mouse xCT/SLC7A11

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Kommentare:

Negative Control: (ABIN7582046)

Blocking Peptide: (ABIN7237192)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 15 μL or 50 μL double distilled water (DDW), depending on the sample size.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu SLC7A11

Target: SLC7A11 (Solute Carrier Family 7, (Cationic Amino Acid Transporter, Y+ System) Member 11 (SLC7A11))

Andere Bezeichnung: SLC7A11

Hintergrund: Cystine/glutamate transporter, Amino acid transport system xc-, Solute carrier family 7 member 11, Calcium channel blocker resistance protein CCBR1,The SLC7 family is divided into two subgroups, the cationic amino acid transporters (the CAT family, SLC7A1-4) and the glycoprotein-associated amino acid transporters (the gpaAT family, SLC7A5-11) also called light chains or catalytic chains of the hetero(di)meric amino acid transporters (HAT). The gene SLC7A11 encodes the xCT protein (also known as Cystine/glutamate transporter).xCT, as well as other HATs, is comprised of a light chain with 12 non-glycosylated transmembrane helices and is associated with the glycosylated heavy chain 4F2hc (CD98, SLC3A2) or rBAT (SLC3A1) through a conserved disulphide bridge. The COOH-terminus of the xCT is localized intracellularly. Association of the light and heavy chains is required for surface expression of the transporter which forms the heterodimeric amino acid transport system xc-. xCT is mainly expressed macrophages, brain, retinal pigment cells, liver and kidney. xCT expression is elevated in cells requiring high glutathione synthesis, such as activated macrophages, neuronal and glial cells and other cells after glutathione depletion1.xCT operates in a Na+-independent and electroneutral mode, exchanging extracellular anionic cystine (with pH dependence) for glutamate with a stoichiometry of 1:1. The driving force for this exchange is generated by the cystine concentration gradient (intracellular reduction) and the high intracellular concentration of glutamate.xCT has been suggested as a mediator of treatment resistance in cancer patients. In tumor cells, xCT plays a crucial role in regulating intracellular levels of glutathione which has been broadly implicated in resistance to chemotherapy2.

Alternative names: Cystine/glutamate transporter, Amino acid transport system xc-, Solute carrier family 7 member 11, Calcium channel blocker resistance protein CCBR1

Gen-ID: 26570

NCBI Accession: NM_014331

UniProt: Q9WTR6