SLC7A11 Antikörper

Produktnummer ABIN6655337

Hersteller Produkt- Nr.: 600-401-gu3s

Zitiert in 1 Publikation. Der Kaninchen Polyklonal anti-SLC7A11 Antikörper (ABIN6655337) detektiert spezifisch SLC7A11 in WB, IHC, ELISA, IP, FACS, IF und FM. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für SLC7A11 Antikörper (ABIN6655337)

Target: SLC7A11 (Solute Carrier Family 7, (Cationic Amino Acid Transporter, Y+ System) Member 11 (SLC7A11))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SLC7A11 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Produktdetails anti-SLC7A11 Antikörper

Hersteller Produkt- Nr.: 600-401-gu3s

Hersteller: Rockland

Verwendungszweck: xCT Antibody

Kreuzreaktivität (Details): This affinity purified antibody is directed against human xCT.

Aufreinigung: This product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterilität: Sterile filtered

Immunogen: Anti-xCT antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human xCT conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunoprecipitation_Dilution: User Optimized

ELISA_Dilution: 5 μg/mL

Immunohistochemistry_Dilution: 1:200

Flow_Cytometry_Dilution: 1:200

IF_Microscopy_Dilution: 10 μg/mL

Western_Blot_Dilution: 1:1000

Kommentare:

Anti-xCT Antibody has been tested in Western Blot, ELISA, Immunofluorescence, and flow cytometry. Expect a band at 55.4 kDa in western blot using appropriate lysates. Positive control used HeLa, NIH-3T3, A549, HCT-116, or PC3 whole cell lysates at 0.1-1.0μg/mL for WB and PC3 with MeOH at 10μg/mL in IF. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Referenzen für anti-SLC7A11 Antikörper (ABIN6655337)

Tamiya, Urushihara, Shizuma, Ogawa, Nakai, Wakamatsu, Takenaka, Kakunaga: "SHARPIN Enhances Ferroptosis in Synovial Sarcoma Cells via NF-κB- and PRMT5-Mediated PGC1α Reduction." in: Cancers, Vol. 15, Issue 13, (2023) (PubMed).

Details zu SLC7A11

Target: SLC7A11 (Solute Carrier Family 7, (Cationic Amino Acid Transporter, Y+ System) Member 11 (SLC7A11))

Andere Bezeichnung: xCT

Hintergrund:

Synonyms: rabbit anti-xCT antibody, SLC7A11, Cystine/glutamate transporter, Amino acid transport system xc-, Calcium channel blocker resistance protein CCBR1, Solute carrier family 7 member 11

Background: xCT belongs to the amino acid-polyamine-organocation (APC) superfamily and L-type amino acid transporter (LAT) family. xCT gene encodes a member of a heteromeric, sodium-independent, anionic amino acid transport system that is highly specific for cysteine and glutamate. In this system, designated Xc(-), the anionic form of cysteine is transported in exchange for glutamate. Increased expression of this gene results in neuronal cell death. xCT may be associated with kaposi sarcoma, dyscalculia, cystinuria, spondylolsis, and anemia of prematurity. Anti-xCT Antibody is useful for researchers interested in Cancer Research, Glucose/Energy Metabolism Research, and Cell Surface Protein Interaction Research.

Gene Name: SLC7A11

Gen-ID: 23657

NCBI Accession: NP_055146

UniProt: Q9UPY5