SLC5A8 Antikörper (Intracellular)

Produktnummer ABIN7043734

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch SLC5A8 in WB und IHC. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für SLC5A8 Antikörper (Intracellular) (ABIN7043734)

Target: SLC5A8 (Solute Carrier Family 5 (Iodide Transporter), Member 8 (SLC5A8))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SLC5A8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-SLC5A8 Antikörper

Bindungsspezifität: AA 544-558, Intracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to SMCT1 (SLC5A8) Transporter

Spezifität: Intracellular, C-terminus.

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Mouse,rat - 13 out of 15 amino acid residues identical

Produktmerkmale: Anti-SMCT1 (SLC5A8) Antibody (ABIN7043734, ABIN7044495 and ABIN7044496) is a highly specific antibody directed against an epitope of the human SLC5A8. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize SLC5A8 from mouse, rat and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: Peptide (C)NGSRAPDHDVTQERDE, corresponding to amino acid residues 15-30 of  mouse beta-2-Adrenoceptor

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Kommentare:

Negative Control: (ABIN7237129)

Blocking Peptide: (ABIN7237129)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu SLC5A8

Target: SLC5A8 (Solute Carrier Family 5 (Iodide Transporter), Member 8 (SLC5A8))

Andere Bezeichnung: SLC5A8

Hintergrund: Sodium-Coupled Monocarboxylate Transporter 1, Solute Carrier Family 5 Member 8, Apical Iodide Transporter, AIT,Sodium-coupled monocarboxylate transporter 1, SLC5A8, is an electrogenic sodium (Na+) and chloride (Cl-)-dependent sodium-coupled solute transporter that belongs to the Na+/glucose co-transporter gene family (SLC5) 1.It is expressed in the colon, ileum, kidney and thyroid gland and it is involved in the transport of a variety of monocarboxylates (short-chain fatty acids including L-lactate, D-lactate, pyruvate, acetate, propionate, valerate and butyrate), lactate, mocarboxylate drugs (nicotinate, benzoate, salicylate and 5-aminosalicylate) and ketone bodies (beta-D-hydroxybutyrate, acetoacetate and alpha-ketoisocaproate) 1.SLC5A8 was found to be silenced in colon cancer, gastric cancer and possibly other cancer types and hence it is a candidate tumor suppressor gene. This is probably mediated through its role in transforming butyrate, a metabolite that is related to these types of cancer 2.The structure of SLC5A8 was predicted based on its homology to sodium-iodide symporter (NIS) and on the fact that it is a part of the Solut Sodium Symporter (SSS) protein family. The structure contains 13 α-helical TM segments, with the N-terminus facing the extracellular medium and the C-terminus facing the cytoplasm, and a conserved sodium binding site 3.

Alternative names: Sodium-Coupled Monocarboxylate Transporter 1, Solute Carrier Family 5 Member 8, Apical Iodide Transporter, AIT

Gen-ID: 160728

NCBI Accession: NM_145913

UniProt: Q8N695