Serotonin Receptor 3A Antikörper (2nd Cytoplasmic Loop)

Produktnummer ABIN7043621

Der Kaninchen Polyklonal anti-Serotonin Receptor 3A Antikörper wird verwendet zum Nachweis von Serotonin Receptor 3A in Proben von Ratte. Er wurde validiert für WB, IHC und IF.

Kurzübersicht für Serotonin Receptor 3A Antikörper (2nd Cytoplasmic Loop) (ABIN7043621)

Target: Serotonin Receptor 3A (HTR3A)

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Serotonin Receptor 3A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-Serotonin Receptor 3A Antikörper

Bindungsspezifität: 2nd Cytoplasmic Loop, AA 342-355

Verwendungszweck: A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 3A

Kreuzreaktivität: Maus, Ratte

Homologie: Mouse - identical, human - 13,14 amino acid residues identical

Produktmerkmale: Anti-5HT3A Receptor (HTR3A) Antibody (ABIN7043621, ABIN7045283 and ABIN7045284) is a highly specific antibody directed against an epitope of rat Serotonin receptor 3A. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize 5-HT3A from mouse, human, and rat samples. 

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)RPVPDWLRHLVLDR, corresponding to amino acid residues 342-355 of rat 5-Hydroxytryptamine receptor 3A

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:400

IP: The optimal concentration should be determined by the user

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu Serotonin Receptor 3A

Target: Serotonin Receptor 3A (HTR3A)

Andere Bezeichnung: HTR3A

Hintergrund:

Synonyms: 5-Hydroxytryptamine receptor 3A, Serotonin receptor 3A, Serotonin-gated ion channel receptor 3A

Description: 5-Hydroxytryptamine receptor 3A (5-HT3A, HTR3A) belongs to the superfamily of ligand-gated ion channels. Serotonin receptors, other than the 5-HT3 subtype, belong to the superfamily of G-protein coupled receptors.The 5-HT3 receptor is formed by five subunits arranged around a pore forming unit. Receptors could be either monomeric, such as 5-HT3A or heteromeric entities like 5-HT3A/B. Indeed, the type of channel formed displays different pharmacological and electrophysiological characteristics1,2. To date, five 5-HT3 subunits have been identified and labeled 5-HT3A-E, which show variability in the N-terminus and in the transmembrane region2. 5-HT3A and 5-HT3B are the best characterized among the different types.In general, 5-HT3 receptors are located in the peripheral and central nervous system, in lymphocytes and intestinal enterochromaffine cells2. In presynaptic neurons, activation of these receptors leads to an increase in intracellular Ca2+ (by both influx and mobilization of intracellular stores), and modulates the release of a number of neurotransmitters and neuropeptides2. At the postsynaptic level, activation leads to membrane depolarization2,3. The 5-HT3A subunit is expressed in GABAergic and enkephalinergic neurons in the spinal dorsal horn thereby marking its possible antinociceptive effect4.5-HT3 receptors have become important targets for which to develop treatments for irritable bowel syndrome (IBS), side effects resulting from chemotherapeutic treatment, schizophrenia and bipolar disorder1,2.

Gen-ID: 79246

NCBI Accession: NM_000869

UniProt: P35563

Pathways: Synaptic Membrane