Serotonin Receptor 2B Antikörper (Extracellular, N-Term)

Produktnummer ABIN7887154

Der Kaninchen Polyklonal anti-Serotonin Receptor 2B Antikörper (ABIN7887154) detektiert spezifisch Serotonin Receptor 2B in WB, IHC, FACS und LCI. Dieser Antikörper reagiert spezifisch mit Proben aus Ratte.

Kurzübersicht für Serotonin Receptor 2B Antikörper (Extracellular, N-Term) (ABIN7887154)

Target: Serotonin Receptor 2B (HTR2B)

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Serotonin Receptor 2B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Produktdetails anti-Serotonin Receptor 2B Antikörper

Bindungsspezifität: AA 6-20, Extracellular, N-Term

Verwendungszweck: A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 2B

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Mouse - identical,human - 11,15 amino acid residues identical

Produktmerkmale: Anti-5HT2B Receptor (HTR2B) (extracellular) Antibody (ABIN7043619, ABIN7045288 and ABIN7045289) is a highly specific antibody directed against an epitope of the rat 5-B receptor. The antibody can be used in western blot and immunohistochemistry applications. It recognizes an extracellular epitope and can potentially detect 5HT2B receptor in living cells. It has been designed to recognize 5-hydroxytryptamine receptor 2B from rat, mouse and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)KMSEQ STISEHILQK, corresponding to amino acid residues 6-20 of rat 5-Hydroxytryptamine receptor 2B

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: 2.5 mg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:400

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7236792)

Blocking Peptide: (ABIN7236792)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu Serotonin Receptor 2B

Target: Serotonin Receptor 2B (HTR2B)

Andere Bezeichnung: HTR2B

Hintergrund:

Synonyms: 5-Hydroxytryptamine receptor 2B, Serotonin receptor 2B, 5-HT-2B, 5-HT2F, Stomach fundus serotonin receptor

Description: 5-HT is involved in a variety of physiological and biological processes. In the brain, 5-HT has been found to affect sleep, mood, appetite, anxiety, aggression, perception, pain, and cognition1. Signaling of 5-HT is mediated by receptors that are located on the cell membrane of neurons and most other cells in the body. The 5-HT2 family consists of three G-protein coupled receptors (GPCRs): 5-HT2A, 5-HT2B, and 5-HT2C2. Like other members, they are transmembrane proteins consisting of seven membrane-spanning α-helical segments with an extracellular N-terminus and an intracellular C-terminus. The binding of 5-HT to one of its receptors is thought to elicit a conformational change that activates associated heterotrimeric G proteins and recruits other downstream signaling/scaffolding molecules, such as GPCR kinases and β-arrestins.5-HT2B receptors have been found to be present in both rodent and human tissues, particularly in the cardiovascular system, gastrointestinal tract, bone, and central nervous system2. Only low expression levels are found in the brain and blood. The highest expression levels are found in the stomach fundus3.Over-expression of 5-HT2B receptors in hearts of transgenic mice results in cardiac hypertrophy and decreased ventricular function due to enhanced extracellular matrix (ECM) and remodeling. Likewise, genetic deletion of 5-HT2B receptors was shown to lead to ventricular dilation and incomplete cardiac development5.

Gen-ID: 29581

NCBI Accession: NM_000867

UniProt: P30994

Pathways: JAK-STAT Signalweg, Inositol Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of Carbohydrate Metabolic Process