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Presenilin 1 Antikörper (AA 345-359)

Der Kaninchen Polyklonal Anti-Presenilin 1-Antikörper wurde für WB, IHC und IF validiert. Er ist geeignet, Presenilin 1 in Proben von Ratte zu detektieren.
Produktnummer ABIN7043552

Kurzübersicht für Presenilin 1 Antikörper (AA 345-359) (ABIN7043552)

Target

Alle Presenilin 1 (PSEN1) Antikörper anzeigen
Presenilin 1 (PSEN1)

Reaktivität

  • 108
  • 82
  • 59
  • 9
  • 9
  • 8
  • 6
  • 4
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
Ratte

Wirt

  • 98
  • 38
  • 2
  • 2
  • 2
  • 1
Kaninchen

Klonalität

  • 132
  • 11
Polyklonal

Konjugat

  • 63
  • 9
  • 9
  • 6
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser Presenilin 1 Antikörper ist unkonjugiert

Applikation

  • 110
  • 57
  • 52
  • 52
  • 39
  • 18
  • 18
  • 17
  • 13
  • 12
  • 8
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
  • Bindungsspezifität

    • 31
    • 15
    • 15
    • 9
    • 8
    • 8
    • 7
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 345-359

    Verwendungszweck

    A Rabbit Polyclonal Antibody to Presenilin-1

    Spezifität

    3rd cytoplasmic loop (at the Psen1 CTF subunit)

    Kreuzreaktivität

    Human, Maus, Ratte

    Homologie

    Mouse,human - identical

    Produktmerkmale

    Anti-Presenilin-1 Antibody (ABIN7043552, ABIN7044524 and ABIN7044525) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize PSEN1 from rat, mouse, and human samples.

    Aufreinigung

    Affinity purified on immobilized antigen.

    Immunogen

    Immunogen: Synthetic peptide

    Immunogen Sequence: (C)RDSHLGPHRSTPESR, corresponding to amino acid residues 345-359 of rat Psen1

    Isotyp

    IgG
  • Applikationshinweise

    Antigen preadsorption control: 1 μg peptide per 1 μg antibody

    Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:600

    Application Dilutions Western blot wb: 1:500

    Kommentare

    Negative Control: (ABIN7236593)

    Blocking Peptide: (ABIN7236593)

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    0.2 mL double distilled water (DDW).

    Konzentration

    1 mg/mL

    Buffer

    PBS pH 7.4

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

    Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

  • Target

    Presenilin 1 (PSEN1)

    Andere Bezeichnung

    PSEN1

    Hintergrund

    PSEN1, PS-1, Psnl1,Presenilin-1 (PSEN1) is a transmembrane protein encoded by the PS1 gene. The protein is comprised of 9 transmembrane domains. The N- and C-termini of the protein are cytosolic and lumenal respectively. PSEN1, together with three other proteins - nicastrin, presenilin enhancer 2 and anterior pharynx-defective 1 form a protein complex named γ-Secretase. PSEN1 serves as the catalytic subunit of the γ-secretase complex. This complex, along with α- and β-secretases cleaves the amyloid precursor protein (APP). APP is the precursor for β-Amyloid fibrils which are the pathological hallmark of Alzheimer's disease (AD) and mutations in the PSEN1 gene have been implicated in AD pathophysiology. Currently, it remains unclear whether PSEN1 mutations cause disease by a loss of function or a gain of toxic function mechanism1.PS1 mutations causing an overexpression of mutant human PSEN1 also increase the expression of ryanodine receptor 3 in PC12 cells. In addition, PC12 and cortical neuron cells expressing mutant PSEN1 exhibit increased calcium responses to caffeine compared with cells expressing wildtype PSEN1. This enhanced release of calcium is associated with increased cell vulnerability to β-Amyloid and caffeine induced cellular death. It has been hypothesized that PSEN1 and RyR interact directly2.PS1 mutations also enhance inositol triphosphate (IP3)-mediated Ca2+ release in non-excitable and excitable cells. IP3-evoked Ca2+ responses are more than threefold greater in PS1M146V knock-in mice relative to non-transgenic controls. These mutations specifically disrupt intracellular Ca2+ release rather than reduce cytosolic Ca2+ buffering or clearance3.

    Alternative names: Presenilin-1, PSEN1, PS-1, Psnl1

    Gen-ID

    29192

    NCBI Accession

    NM_000021

    UniProt

    P97887

    Pathways

    Notch Signalweg, EGFR Signaling Pathway, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport
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