EPH Receptor A6 Antikörper (Extracellular)

Produktnummer ABIN7043125

Produktdetails anti-EPH Receptor A6 Antikörper

Target: EPH Receptor A6 (Epha6)

Bindungsspezifität: AA 482-495, Extracellular

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EPH Receptor A6 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (Cultured Cells) (IF (cc)), Live Cell Imaging (LCI)

Verwendungszweck: A Rabbit Polyclonal Antibody to EphA6 Receptor

Spezifität: Extracellular, N-terminus

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Mouse,human - identical

Produktmerkmale: Anti-EphA6 (extracellular) Antibody (ABIN7043125, ABIN7044247 and ABIN7044248)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, indirect live cell flow cytometry, and immunohistochemistry applications. It has been designed to recognize EphA6 from rat, mouse, and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KEHEQLTYSSTRSK, corresponding to amino acid residues 482-495 of rat EphA6

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200-1:400

Kommentare:

Negative Control: BLP-ER016

Blocking Peptide: BLP-ER016

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu EPH Receptor A6

Target: EPH Receptor A6 (Epha6)

Andere Bezeichnung: EPHA6 (Epha6 Produkte)

Hintergrund: Ephrin type-A receptor 6, EPH receptor A6, EPH homology kinase 2, EHK-2,EphA6 is an erythropoietin-producing hepatocellular (Eph) receptor that belongs to the tyrosine kinase receptor family. The Eph receptor family consists of 9 EphA receptors and 5 EphB receptors, which serve key roles in normal physiology, including cell migration, cell adhesion, cell proliferation and neuronal synaptic plasticity. These activities depend on the binding of their Eph receptor-interacting (ephrin) proteins, of which include 8 members. Compelling data has demonstrated that Eph receptors and ephrins affect tumor growth, invasiveness, angiogenesis and metastasis1. They are also involved in brain development, learning and memory2.EphA6 signaling pathways are inhibitory for developing axons and blocking these pathways enhances regeneration following spinal cord and brain injury, rendering EphA6 an appealing drug target3.Eph receptors contain an extracellular structure consisting of an ephrin binding domain, the juxtamembrane region connects the extracellular portion of the receptor to the intracellular kinase domain. Two Tyrosine residues on the juxtamembrane region mediate autophosphorylation and receptor dimerization mediates the formation of heterocomplexes that are required for signaling4.

Alternative names: Ephrin type-A receptor 6, EPH receptor A6, EPH homology kinase 2, EHK-2

Gen-ID: 29202

NCBI Accession: NM_001080448

UniProt: P54758

Pathways: RTK Signalweg