CXCR7 Antikörper (Extracellular, N-Term)

Produktnummer ABIN7886252

Der Kaninchen Polyklonal anti-CXCR7 Antikörper (ABIN7886252) detektiert spezifisch CXCR7 in WB, FACS und LCI. Dieser Antikörper reagiert spezifisch mit Proben aus Ratte.

Kurzübersicht für CXCR7 Antikörper (Extracellular, N-Term) (ABIN7886252)

Target: CXCR7 (Chemokine (C-X-C Motif) Receptor 7 (CXCR7))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CXCR7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Produktdetails anti-CXCR7 Antikörper

Bindungsspezifität: AA 2-16, Extracellular, N-Term

Verwendungszweck: A Rabbit Polyclonal Antibody to CXCR7 Chemokine Receptor

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Mouse -14,15 amino acid residues identical, human - 12

Produktmerkmale: Anti-CXCR7 (ACKR3) (extracellular) Antibody (ABIN7043043, ABIN7044181 and ABIN7044182) is a highly specific antibody directed against an epitope of the rat CXCR7 chemokine receptor. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize CXCR7 from human, rat and mouse samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)DVHLFDYVEPGNYSD, corresponding to amino acid residues 2-16 of rat CXCR7 (ACKR3)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:400

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7235067)

Blocking Peptide: (ABIN7235067)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu CXCR7

Target: CXCR7 (Chemokine (C-X-C Motif) Receptor 7 (CXCR7))

Andere Bezeichnung: ACKR3

Hintergrund:

Synonyms: Atypical chemokine receptor 3, C-X-C chemokine receptor type 7, CXCR-7, Chemokine orphan receptor 1, G-protein coupled receptor RDC1 homolog, CMKOR1, GPR159

Description: Chemokines are molecules that control the migration and positioning of the immune system cells. In addition to their function as chemo-attractants they have various other roles in the maturation of immune cells such as T-cell development in the thymus and homeostatic retention of immune cells in the bone marrow1.Chemokines bind to chemokine receptors, members of the G-protein coupled receptor superfamily. They are comprised of seven transmembrane receptors with extracellular N-terminus, three extracellular and three intracellular loops and an intracellular C-terminus. One of the intracellular domains of the receptor couples with G proteins and begins a cascade of signal transduction2.Until recently the CXCR-7 was an "orphan" receptor with its endogenous ligand unknown. This receptor is encoded by the CXCR7/RDC-1 gene which was mapped to mouse chromosome 1 and human chromosome 2. Homology has been shown with the viral gene ORF74 suggesting the receptor might signal without the binding of a ligand. Currently it is known that the chemokines CXCL12 and CXCL11 bind to CXCR-73.CXCR-7 is tightly linked to the regulation of B cell development and differentiation indicating it might have a role in the formation of B memory cells4. CXCR-7 was also found to be associated with tumors where its levels are elevated in many tumor cell lines such as endothelial cells, fetal liver cells and placenta cells5. Despite this suggested role, there is growing evidence that this receptor does not function as a regular GPCR6.

Gen-ID: 84348

NCBI Accession: NM_020311

UniProt: O89039

Pathways: Myometrial Relaxation and Contraction, Negative Regulation of intrinsic apoptotic Signaling