CXCR7 Antikörper (Extracellular)

Produktnummer ABIN7043043

Produktdetails anti-CXCR7 Antikörper

Target: CXCR7 (Chemokine (C-X-C Motif) Receptor 7 (CXCR7))

Bindungsspezifität: AA 2-16, Extracellular

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CXCR7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Verwendungszweck: A Rabbit Polyclonal Antibody to CXCR7 Chemokine Receptor

Spezifität: Extracellular, N-terminus

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Mouse -14,15 amino acid residues identical, human - 12

Produktmerkmale: Anti-CXCR7 (ACKR3) (extracellular) Antibody (ABIN7043043, ABIN7044181 and ABIN7044182) is a highly specific antibody directed against an epitope of the rat CXCR7 chemokine receptor. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize CXCR7 from human, rat and mouse samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DVHLFDYVEPGNYSD, corresponding to amino acid residues 2-16 of rat CXCR7 (ACKR3)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:400

Kommentare:

Negative Control: (ABIN7235067)

Blocking Peptide: (ABIN7235067)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu CXCR7

Target: CXCR7 (Chemokine (C-X-C Motif) Receptor 7 (CXCR7))

Andere Bezeichnung: ACKR3 (CXCR7 Produkte)

Hintergrund: Atypical chemokine receptor 3, C-X-C chemokine receptor type 7, CXCR-7, Chemokine orphan receptor 1, G-protein coupled receptor RDC1 homolog, CMKOR1, GPR159,Chemokines are molecules that control the migration and positioning of the immune system cells. In addition to their function as chemo-attractants they have various other roles in the maturation of immune cells such as T-cell development in the thymus and homeostatic retention of immune cells in the bone marrow1.Chemokines bind to chemokine receptors, members of the G-protein coupled receptor superfamily. They are comprised of seven transmembrane receptors with extracellular N-terminus, three extracellular and three intracellular loops and an intracellular C-terminus. One of the intracellular domains of the receptor couples with G proteins and begins a cascade of signal transduction2.Until recently the CXCR-7 was an "orphan" receptor with its endogenous ligand unknown. This receptor is encoded by the CXCR7/RDC-1 gene which was mapped to mouse chromosome 1 and human chromosome 2. Homology has been shown with the viral gene ORF74 suggesting the receptor might signal without the binding of a ligand. Currently it is known that the chemokines CXCL12 and CXCL11 bind to CXCR-73.CXCR-7 is tightly linked to the regulation of B cell development and differentiation indicating it might have a role in the formation of B memory cells4. CXCR-7 was also found to be associated with tumors where its levels are elevated in many tumor cell lines such as endothelial cells, fetal liver cells and placenta cells5. Despite this suggested role, there is growing evidence that this receptor does not function as a regular GPCR6.

Alternative names: CXCR7 (ACKR3), Atypical chemokine receptor 3, C-X-C chemokine receptor type 7, CXCR-7, Chemokine orphan receptor 1, G-protein coupled receptor RDC1 homolog, CMKOR1, GPR159

Gen-ID: 84348

NCBI Accession: NM_020311

UniProt: O89039

Pathways: Myometrial Relaxation and Contraction, Negative Regulation of intrinsic apoptotic Signaling