BDNF Antikörper (Intracellular)

Produktnummer ABIN7886732

Dieser Meerschweinchen Polyklonal Antikörper detektiert spezifisch BDNF in WB, IHC und IF. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für BDNF Antikörper (Intracellular) (ABIN7886732)

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Reaktivität: Human

Wirt: Meerschweinchen

Klonalität: Polyklonal

Konjugat: Dieser BDNF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-BDNF Antikörper

Bindungsspezifität: AA 166-178, Intracellular

Verwendungszweck: A Guinea Pig Polyclonal Antibody to BDNF

Kreuzreaktivität: Human, Maus, Ratte

Kreuzreaktivität (Details): The antibody is specific for BDNF, it does not crossreact with the closely related neurotrophins NGF, NT-3 or NT-4.

Homologie: Mouse,rat,many other species - identical

Produktmerkmale: Guinea pig Anti-BDNF Antibody is a highly specific antibody directed against an epitope of human brain-derived neurotrophic factor. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize BDNF from rat, human and mouse samples. The antibody is specific for BDNF, it does not crossreact with NGF, or . The antigen used to immunize guinea pigs is the same as Anti-BDNF Antibody (ABIN7042970, ABIN7044761 and ABIN7044762) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)VLEKVPVSKQLK, corresponding to amino acid residues 166-178 of human BDNF (precursor)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:500

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:400

IP: The optimal concentration should be determined by the user

Kommentare:

Positive Control: PCP-B250

Negative Control: (ABIN7234876)

Blocking Peptide: (ABIN7234876)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu BDNF

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Andere Bezeichnung: BDNF

Hintergrund:

Synonyms: Brain-derived neurotrophic factor

Description: Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors which includes nerve growth factor (NGF), neurotrophin-3 (NT-3) and neurotrophin-4/5 (NT-4/5).All neurotrophins are synthesized as preproneurotrophin precursors that are subsequently processed within the intracellular transport pathway to yield proneurotrophins that are further processed to generate the mature form. The mature form of BDNF is a non-covalent stable homodimer that can be secreted in both constitutive and regulated pathways.BDNF conveys its activity by binding to two classes of receptors, a member of the Trk receptor tyrosine kinase family (TrkB) and the pan-neurotrophin receptor p75NTR. Binding of BDNF to the TrkB receptor triggers ligand-induced dimerization and autophosphorylation of tyrosine residues. This activates various signaling cascades such the MAPK, PI3K and PLCγ pathways that are involved in cell growth, survival and differentiation of neurons in the central and peripheral nervous system.Interestingly, recent evidence suggests that BDNF may influence target cell function via ion channel modulation. Ion channel activity in the target cells can be modulated by a TrkB-mediated mechanism that has not yet been determined. BDNF is able to block both KV1.3 and AMPA-subtype glutamate ion channel currents in sensory neurons, while it can induce activation of the TRPC3 cation channel in neurons and of the NaV1.9 Na+ channel in hippocampal neurons. These newly recognized BDNF actions underlie its "rapid" neuronal functions that include changes in neuronal excitability, plasticity and synaptic transmission.

Gen-ID: 627

NCBI Accession: NM_170735

UniProt: P23560

Pathways: RTK Signalweg, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity