Adenosine A2a Receptor Antikörper (Extracellular)

Produktnummer ABIN7042884

Dieses Anti-Adenosine A2a Receptor-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Adenosine A2a Receptor in WB, FACS und LCI. Geeignet für Human.

Kurzübersicht für Adenosine A2a Receptor Antikörper (Extracellular) (ABIN7042884)

Target: Adenosine A2a Receptor (ADORA2A)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Adenosine A2a Receptor Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Produktdetails anti-Adenosine A2a Receptor Antikörper

Bindungsspezifität: AA 146-158, Extracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to Adenosine A2A Receptor

Spezifität: 2nd extracellular loop

Kreuzreaktivität (Details): Will not recognize rat or mouse samples.

Homologie: Human only

Produktmerkmale: Anti-Human Adenosine A2A Receptor (extracellular) Antibody (ABIN7042884, ABIN7043896 and ABIN7043897) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize A2AR from human samples only. The antibody will not recognize the receptor from mouse or rat samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CGQPKEGKNHSQG, corresponding to amino acid residues 146 - 158 of human adenosine A2A receptor

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu Adenosine A2a Receptor

Target: Adenosine A2a Receptor (ADORA2A)

Andere Bezeichnung: ADORA2A

Hintergrund: Adenosine receptor A2A, A2AR, ADORA2A,Adenosine is an endogenous nucleoside generated locally in tissues under conditions of hypoxia, ischemia, or inflammation. It modulates a variety of physiological functions in many tissues including the brain and heart1,2. Adenosine exerts its actions via four specific adenosine receptors (also named P1 purinergic receptors): Adenosine A1 Receptor (A1AR), Adenosine A2A Receptor (A2AAR), Adenosine A2B Receptor (A2BAR), and Adenosine A3 Receptor (A3AR). All are integral membrane proteins and are members of the G protein-coupled receptor superfamily. They share a common structure of seven putative transmembrane domains, an extracellular -NH2 terminus, cytoplasmic -COOH terminus, and a third intracellular loop important for binding G proteins.1-3 The adenosine receptors can be distinguished on the basis of their differential selectivity for adenosine analogs1-3.Adenosine receptors control neurotransmitter release through the facilitatory A2AAR and the inhibitory A1AR.4 A2AAR and A1AR are the major adenosine receptor subtypes expressed in the central nervous system (CNS). A2AAR is mainly expressed in the striatum on GABAergic striatopallidal neurons, while A1AR is widely distributed throughout the CNS5,6.A2AAR was suggested to play a critical role in attenuation of systemic inflammatory responses and prevention of extensive tissue damage.7 It was suggested that extracellular adenosine that accumulates in inflamed and damaged tissue may activate the A2AAR expressed in immune cells leading to termination/inhibition of the immune response.7 It was further suggested that this same mechanism may protect tumors from antitumor T cells through an immunosuppressive signal generated by the activation of A2AAR on T cells by extracellular adenosine produced from hypoxic cancerous tissues8.

Alternative names: A2A Adenosine Receptor, Adenosine Receptor A2A, A2AR, ADORA2A

Gen-ID: 135

NCBI Accession: NM_000675

UniProt: P29274

Pathways: Neurotrophin Signalübertragung, cAMP Metabolic Process, Synaptic Membrane, Feeding Behaviour, Cancer Immune Checkpoints