Adenosine A2a Receptor Antikörper (3rd Intracellular Loop)

Produktnummer ABIN7042883

Der Kaninchen Polyklonal anti-Adenosine A2a Receptor Antikörper wird verwendet zum Nachweis von Adenosine A2a Receptor in Proben von Maus. Er wurde validiert für WB, IHC, IF und IC.

Kurzübersicht für Adenosine A2a Receptor Antikörper (3rd Intracellular Loop) (ABIN7042883)

Target: Adenosine A2a Receptor (ADORA2A)

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Adenosine A2a Receptor Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Güteklasse: KO Validated

Produktdetails anti-Adenosine A2a Receptor Antikörper

Bindungsspezifität: 3rd Intracellular Loop, AA 201-215

Verwendungszweck: A Rabbit Polyclonal Antibody to Adenosine A2A Receptor

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Rat,human,and canis - identical

Produktmerkmale: Anti-Adenosine A2A Receptor Antibody (ABIN7042883, ABIN7043888 and ABIN7043889) is a highly specific antibody directed against an epitope of mouse protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize A2aR from human, rat, and mouse samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)RQLKQMESQPLPGER, corresponding to amino acid residues 201-215 of mouse adenosine A2A receptor

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:50

IP: The optimal concentration should be determined by the user

Kommentare:

Cited Application: IP|IHC

Negative Control: (ABIN7234626)

Blocking Peptide: (ABIN7234626)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu Adenosine A2a Receptor

Target: Adenosine A2a Receptor (ADORA2A)

Andere Bezeichnung: ADORA2A

Hintergrund:

Synonyms: ADORA2A, A2AAR, Adenosine A2aR

Description: Adenosine is an endogenous nucleoside generated locally in tissues under conditions of hypoxia, ischemia, or inflammation. It modulates a variety of physiological functions in many tissues including the brain and heart.1,2 Adenosine exerts its actions via four specific adenosine receptors (also named P1 purinergic receptors): Adenosine A1 Receptor (A1AR), Adenosine A2A Receptor (A2AAR), Adenosine A2B Receptor (A2BAR), and Adenosine A3 Receptor (A3AR). All are integral membrane proteins and are members of the G protein-coupled receptor superfamily. They share a common structure of seven putative transmembrane domains, an extracellular -NH2 terminus, cytoplasmic -COOH terminus, and a third intracellular loop important for binding G proteins.1-3 The adenosine receptors can be distinguished on the basis of their differential selectivity for adenosine analogs.1-3Adenosine receptors control neurotransmitter release through the facilitatory A2AAR and the inhibitory A1AR.4 A2AAR and A1AR are the major adenosine receptor subtypes expressed in the central nervous system (CNS). A2AAR is mainly expressed in the striatum on GABAergic striatopallidal neurons, while A1AR is widely distributed throughout the CNS.5,6A2AAR was suggested to play a critical role in attenuation of systemic inflammatory responses and prevention of extensive tissue damage.7 It was suggested that extracellular adenosine that accumulates in inflamed and damaged tissue may activate the A2AAR expressed in immune cells leading to termination/inhibition of the immune response.7 It was further suggested that this same mechanism may protect tumors from antitumor T cells through an immunosuppressive signal generated by the activation of A2AAR on T cells by extracellular adenosine produced from hypoxic cancerous tissues.8

Gen-ID: 11540

NCBI Accession: NM_000675

UniProt: Q60613

Pathways: Neurotrophin Signalübertragung, cAMP Metabolic Process, Synaptic Membrane, Feeding Behaviour, Cancer Immune Checkpoints