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EGLN1 Antikörper (AA 42-140)

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch EGLN1 in WB, ELISA, IF (cc), IF (p), IHC (p) und IHC (fro). Er zeigt eine Reaktivität gegenüber Human und Maus.
Produktnummer ABIN702640

Kurzübersicht für EGLN1 Antikörper (AA 42-140) (ABIN702640)

Target

Alle EGLN1 Antikörper anzeigen
EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))

Reaktivität

  • 67
  • 31
  • 15
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Human, Maus

Wirt

  • 51
  • 17
  • 2
Kaninchen

Klonalität

  • 53
  • 17
Polyklonal

Konjugat

  • 38
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser EGLN1 Antikörper ist unkonjugiert

Applikation

  • 52
  • 19
  • 17
  • 13
  • 12
  • 9
  • 6
  • 6
  • 4
  • 3
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • Bindungsspezifität

    • 14
    • 12
    • 6
    • 5
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 42-140

    Kreuzreaktivität

    Human, Maus

    Homologie

    Cow,Pig,Rabbit

    Aufreinigung

    Purified by Protein A.

    Immunogen

    KLH conjugated synthetic peptide derived from human PHD2

    Isotyp

    IgG
  • Applikationshinweise

    WB 1:300-5000
    ELISA 1:500-1000
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    1 μg/μL

    Buffer

    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

    Konservierungsmittel

    ProClin

    Vorsichtsmaßnahmen

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

    Haltbarkeit

    12 months
  • Target

    EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))

    Andere Bezeichnung

    PHD2

    Hintergrund

    Synonyms: HPH2, PHD2, SM20, ECYT3, HPH-2, HIFPH2, ZMYND6, C1orf12, HIF-PH2, Egl nine homolog 1, Hypoxia-inducible factor prolyl hydroxylase 2, HIF-prolyl hydroxylase 2, Prolyl hydroxylase domain-containing protein 2, SM-20, EGLN1, PNAS-118, PNAS-137

    Background: Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF1B. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN1 is the most important isozyme under normoxia and, through regulating the stability of HIF1, involved in various hypoxia-influenced processes such as angiogenesis in retinal and cardiac functionality. Target proteins are preferencially recognized via a LXXLAP motif.

    Gen-ID

    54583

    UniProt

    Q9GZT9

    Pathways

    cAMP Metabolic Process, Warburg Effekt
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