EGLN1 Antikörper (AA 42-140)

Produktnummer ABIN702640

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch EGLN1 in WB, ELISA, IF (cc), IF (p), IHC (p) und IHC (fro). Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für EGLN1 Antikörper (AA 42-140) (ABIN702640)

Target: EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EGLN1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-EGLN1 Antikörper

Bindungsspezifität: AA 42-140

Kreuzreaktivität: Human, Maus

Homologie: Cow,Pig,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PHD2

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu EGLN1

Target: EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))

Andere Bezeichnung: PHD2

Hintergrund:

Synonyms: HPH2, PHD2, SM20, ECYT3, HPH-2, HIFPH2, ZMYND6, C1orf12, HIF-PH2, Egl nine homolog 1, Hypoxia-inducible factor prolyl hydroxylase 2, HIF-prolyl hydroxylase 2, Prolyl hydroxylase domain-containing protein 2, SM-20, EGLN1, PNAS-118, PNAS-137

Background: Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF1B. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN1 is the most important isozyme under normoxia and, through regulating the stability of HIF1, involved in various hypoxia-influenced processes such as angiogenesis in retinal and cardiac functionality. Target proteins are preferencially recognized via a LXXLAP motif.

Gen-ID: 54583

UniProt: Q9GZT9

Pathways: cAMP Metabolic Process, Warburg Effekt