CTNNB1 Antikörper (Internal Region)

Produktnummer ABIN7013874

Dieser Ziege Polyklonal Antikörper detektiert spezifisch CTNNB1 in WB, ELISA, IF und FACS. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für CTNNB1 Antikörper (Internal Region) (ABIN7013874)

Target: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser CTNNB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-CTNNB1 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: CTNNB1 / catenin beta-1

Sequenz: ETARCTAGTL HNLS

Spezifität: Reported variants represent identical protein: NP_001091679.1, NP_001895.1, NP_001091680.1.

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Schwein, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 90 kDa band observed in A431 cell lysates-and in preliminary testing of CACO2 cell lysate (calculated MW of 85.5 kDa according to NP_001895.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and membranes of U251 and A431 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of U251 cells. Recommended c

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu CTNNB1

Target: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Andere Bezeichnung: CTNNB1

Hintergrund: CTNNB1, catenin (cadherin-associated protein), beta 1, 88 kDa, CTNNB, MRD19, armadillo, catenin beta-1

Gen-ID: 1499, 12387, 84353

Pathways: WNT Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Tube Formation, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2