CTNNB1 Antikörper (Internal Region)

Produktnummer ABIN7013874

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch CTNNB1 in WB, ELISA, IF und FACS. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für CTNNB1 Antikörper (Internal Region) (ABIN7013874)

Target: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser CTNNB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-CTNNB1 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: CTNNB1 / catenin beta-1

Sequenz: ETARCTAGTL HNLS

Spezifität: Reported variants represent identical protein: NP_001091679.1, NP_001895.1, NP_001091680.1.

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Schwein, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 90 kDa band observed in A431 cell lysates-and in preliminary testing of CACO2 cell lysate (calculated MW of 85.5 kDa according to NP_001895.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and membranes of U251 and A431 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of U251 cells. Recommended c

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu CTNNB1

Target: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Andere Bezeichnung: CTNNB1

Hintergrund: CTNNB1, catenin (cadherin-associated protein), beta 1, 88 kDa, CTNNB, MRD19, armadillo, catenin beta-1

Gen-ID: 1499, 12387, 84353

Pathways: WNT Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Tube Formation, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2