Hexokinase 2 Antikörper (AA 605-868)

Produktnummer ABIN7438772

Produktdetails anti-Hexokinase 2 Antikörper

Target: Hexokinase 2 (HK2)

Bindungsspezifität: AA 605-868

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Hexokinase 2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Verwendungszweck: Polyclonal Antibody to Hexokinase 2 (HK2)

Spezifität: The antibody is a rabbit polyclonal antibody raised against HK2. It has been selected for its ability to recognize HK2 in immunohistochemical staining and western blotting.

Kreuzreaktivität: Human, Ratte

Aufreinigung: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Hexokinase 2 (HK2) corresdonding to Pro605~His868 (Accession # O08528) with N-terminal His and GST Tag

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 0.5-2 μg/mLImmunocytochemistry in formalin fixed cells: 5-20 μg/mLImmunohistochemistry in formalin fixed frozen section: 5-20 μg/mLImmunohistochemistry in paraffin section: 5-20 μg/mLEnzyme-linked Immunosorbent Assay: 0.05-2 μg/mLOptimal working dilutions must be determined by end user.

Kommentare:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Konservierungsmittel: ProClin, Sodium azide

Vorsichtsmaßnahmen: This product contains ProClin and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 24 months

Details zu Hexokinase 2

Target: Hexokinase 2 (HK2)

Andere Bezeichnung: Hexokinase 2 (HK2 Produkte)

Hintergrund: HK II, Muscle form hexokinase

UniProt: O08528

Pathways: PI3K-Akt Signalweg, Carbohydrate Homeostasis, Warburg Effekt