PDPK1 Antikörper (AA 172-399)

Produktnummer ABIN7440905

Der Kaninchen Polyklonal Anti-PDPK1 Antikörper wurde für WB, IHC, ICC und IP validiert. Er ist geeignet, PDPK1 in Proben von Ratte zu detektieren.

Kurzübersicht für PDPK1 Antikörper (AA 172-399) (ABIN7440905)

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PDPK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Produktdetails anti-PDPK1 Antikörper

Bindungsspezifität: AA 172-399

Verwendungszweck: Polyclonal Antibody to Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1)

Spezifität: The antibody is a rabbit polyclonal antibody raised against PDK1. It has been selected for its ability to recognize PDK1 in immunohistochemical staining and western blotting.

Aufreinigung: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1) corresdonding to Val172~Pro399 with N-terminal His Tag

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 0.5-2 μg/mL,1:250-1000Immunohistochemistry: 5-20 μg/mL,1:25-100Immunocytochemistry: 5-20 μg/mL,1:25-100Optimal working dilutions must be determined by end user.

Kommentare:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.51 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 24 months

Details zu PDPK1

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Andere Bezeichnung: PDK1

Hintergrund: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial

UniProt: Q63065

Pathways: PI3K-Akt Signalweg, T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals