GRK2 Antikörper (AA 398-640)

Produktnummer ABIN7440779

Der Kaninchen Polyklonal Anti-GRK2-Antikörper wurde für WB, IHC, ICC und IP validiert. Er ist geeignet, GRK2 in Proben von Human zu detektieren.

Kurzübersicht für GRK2 Antikörper (AA 398-640) (ABIN7440779)

Target: GRK2 (ADRBK1) (Adrenergic, Beta, Receptor Kinase 1 (ADRBK1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GRK2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Produktdetails anti-GRK2 Antikörper

Bindungsspezifität: AA 398-640

Verwendungszweck: Polyclonal Antibody to Beta Adrenergic Receptor Kinase (BARK)

Spezifität: The antibody is a rabbit polyclonal antibody raised against BARK. It has been selected for its ability to recognize BARK in immunohistochemical staining and western blotting.

Kreuzreaktivität: Maus

Aufreinigung: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Beta Adrenergic Receptor Kinase (BARK) corresdonding to Asp398~Leu640 (Accession # P25098) with N-terminal His and GST Tag

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1-5 μg/mLImmunocytochemistry in formalin fixed cells: 5-20 μg/mLImmunohistochemistry in formalin fixed frozen section: 5-20 μg/mLImmunohistochemistry in paraffin section: 5-20 μg/mLEnzyme-linked Immunosorbent Assay: 0.05-2 μg/mLOptimal working dilutions must be determined by end user.

Kommentare:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 24 months

Details zu GRK2

Target: GRK2 (ADRBK1) (Adrenergic, Beta, Receptor Kinase 1 (ADRBK1))

Andere Bezeichnung: BARK

Hintergrund: ADRbK1, B-ARK1, bARK1, Beta-ARK1, GRK2, G-protein coupled receptor kinase 2, Adrenergic Receptor Beta Kinase 1

UniProt: P25098

Pathways: EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling