GRK2 Antikörper (C-Term)

Produktnummer ABIN6655845

Produktdetails anti-GRK2 Antikörper

Target: GRK2 (ADRBK1) (Adrenergic, Beta, Receptor Kinase 1 (ADRBK1))

Bindungsspezifität: C-Term

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser GRK2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Kreuzreaktivität: Human, Maus

Aufreinigung: This affinity purified antibody is directed against human GRK2. This product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis of the sequence shows 100% reactivity to human, and 90% to rat, mouse, and golden hamster.

Immunogen:

Immunogen: Anti-GRK2 antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to a near C-terminal portion of human GRK2 conjugated to Keyhole Limpet Hemocyanin (KLH).

Immunogen Type: Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:1000

Application Note: Anti-GRK2 Antibody has been tested in ELISA, Western Blot, and IHC. Expect a band at ~79.6 kDa in western blot using appropriate lysates. Positive control used: Human high grade lymphoma tissue in Immunohistochemistry and THP-1 lysate in western blot.

ELISA Dilution: 1:10,000-1:50,000

Western Blot Dilution: 1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Details zu GRK2

Target: GRK2 (ADRBK1) (Adrenergic, Beta, Receptor Kinase 1 (ADRBK1))

Andere Bezeichnung: GRK2 (ADRBK1 Produkte)

Synonyme: Adrbk-1 antikoerper, Bark-1 antikoerper, GRK2 antikoerper, betaARK1 antikoerper, BARK1 antikoerper, BETA-ARK1 antikoerper, GRK-2 antikoerper, Grk2 antikoerper, beta-adrenergic receptor kinase 1 antikoerper, G protein-coupled receptor kinase 2 antikoerper, Tsp_00497 antikoerper, grk2 antikoerper, Grk2 antikoerper, GRK2 antikoerper

Hintergrund:

Synonyms: Goat Anti-G Protein-Coupled Receptor Kinase 2 Antibody, Goat Anti-GRK2 Antibody, Beta-ARK-1, ADRBK1, BARK1, G-Protein Coupled Receptor Kinase 2, Adrenergic, Beta, Receptor Kinase 1, Adrenergic Beta Receptor Kinase 1, Beta-Adrenergic Receptor Kinase 1, EC 2.7.11.15, BETA-ARK1, BARK

Background: GRK2 (G Protein-Coupled Receptor Kinase 2) is a member of the G protein-coupled receptor kinase family of proteins. GRK2 Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and as well as a wide range of other substrates including non-GPCR cell surface receptors, and cytoskeletal, mitochondrial, and transcription factor proteins. It is a key regulator of LPAR1 signaling. GRK2 competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. It desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner. And positively regulates ciliary smoothened (SMO)-dependent Hedgehog (Hh) signaling pathway by facilitating the trafficking of SMO into the cilium and the stimulation of SMO activity. Data from rodent models supports a role for this gene in embryonic development, heart function and metabolism. Elevated expression of this gene has been observed in human patients with heart failure and Alzheimer's disease. Anti-GRK2 Antibody is useful for researchers interested in Neuroscience, Cytokines & Growth Factors, and Stem Cell Research.

Gene Name: GRK2

Gen-ID: 156

NCBI Accession: NP_001610

UniProt: P25098

Pathways: EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling