ENOS Antikörper (AA 519-702)

Produktnummer ABIN7432802

Der Kaninchen Polyklonal Anti-ENOS-Antikörper wurde für WB, IHC, ICC und IP validiert. Er ist geeignet, ENOS in Proben von Ratte zu detektieren.

Kurzübersicht für ENOS Antikörper (AA 519-702) (ABIN7432802)

Target: ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ENOS Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Produktdetails anti-ENOS Antikörper

Bindungsspezifität: AA 519-702

Verwendungszweck: Polyclonal Antibody to Endothelial NOS (eNOS)

Spezifität: The antibody is a rabbit polyclonal antibody raised against eNOS. It has been selected for its ability to recognize eNOS in immunohistochemical staining and western blotting.

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Schwein, Kaninchen

Aufreinigung: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Endothelial NOS (eNOS) corresdonding to Ala519~Phe702 with N-terminal His Tag

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 0.01-3 μg/mL,Immunohistochemistry: 5-30 μg/mL,Immunocytochemistry: 5-30 μg/mL,Optimal working dilutions must be determined by end user.

Kommentare:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 24 months

Details zu ENOS

Target: ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

Andere Bezeichnung: eNOS

Hintergrund: NOS3, ECNOS, NOS III, E-NOS, C-NOS, ENOS, CNOS, Constitutive NOS, Nitric Oxide Synthase 3, Endothelial

UniProt: Q62600

Pathways: ACE Inhibitor Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Response to Molecule of Bacterial Origin, Myometrial Relaxation and Contraction, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling