Hexokinase 1 Antikörper (C-Term)

Produktnummer ABIN6992150

Der Kaninchen Polyklonal Anti-Hexokinase 1-Antikörper wurde für WB, ELISA, IF und IHC (p) validiert. Er ist geeignet, Hexokinase 1 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für Hexokinase 1 Antikörper (C-Term) (ABIN6992150)

Target: Hexokinase 1 (HK1)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Hexokinase 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Hexokinase 1 Antikörper

Bindungsspezifität: AA 830-880, C-Term

Spezifität: Hexokinase 1 antibody is human, mouse and rat reactive. Multiple isoforms of Hexokinase 1 are known to exist.

Aufreinigung: Hexokinase 1 antibody is affinity chromatography purified via peptide column.

Immunogen: Hexokinase 1 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human Hexokinase 1. The immunogen is located within amino acids 830 - 880 of Hexokinase 1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Hexokinase 1 antibody can be used for detection of Hexokinase 1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in rat samples, Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Hexokinase 1 antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Hexokinase 1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Details zu Hexokinase 1

Target: Hexokinase 1 (HK1)

Andere Bezeichnung: Hexokinase 1

Hintergrund: There are four major glucose-phosphorylating isoenzymes, designated Hexokinase 1 I, II, III, and IV (1). Hexokinase 1 activity is involved in the first step in several metabolic pathways including phosphorylation of glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway (1,2). Hexokinase 1 2 is the predominant Hexokinase 1 isozyme expressed in insulin-responsive tissues such as skeletal muscle and its expression is insulin-responsive (3). It is involved in the increased rate of glycolysis seen in rapidly growing cancer cells (4).

Molekulargewicht: Predicted: 101 kDa

Observed: 103 kDa

Gen-ID: 3098

NCBI Accession: NP_000179

UniProt: P19367

Pathways: Carbohydrate Homeostasis, Warburg Effekt