Rubicon Antikörper (N-Term)

Produktnummer ABIN6992053

Produktdetails anti-Rubicon Antikörper

Target: Rubicon (KIAA0226) (KIAA0226)

Bindungsspezifität: AA 240-290, N-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Rubicon Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: Rubicon antibody is human, mouse and rat reactive. Multiple isoforms of Rubicon are known to exist.

Aufreinigung: Rubicon antibody is affinity chromatography purified via peptide column.

Immunogen: Rubicon antibody was raised against a 16 amino acid peptide near the amino terminus of human Rubicon. The immunogen is located within amino acids 240 - 290 of Rubicon.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Rubicon body can be used for detection of Rubicon by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rubicon antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Rubicon antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Details zu Rubicon

Target: Rubicon (KIAA0226) (KIAA0226)

Andere Bezeichnung: Rubicon (KIAA0226 Produkte)

Hintergrund: Two Beclin-1-interacting proteins, the run domain Beclin-1 interacting and cysteine-rich containing protein (Rubicon) and ATG14L, reciprocally regulate autophagy at different stages. Knockdown of Rubicon caused enhancement of autophagy while that of ATG14L caused a defect in autophagosome formation (1). Rubicon functions as part of a Beclin-1-PIK3C3-containing autophagy complex and is also an essential, positive regulator of the NAPDH oxidase complex (2). Upon microbial infection or TLR2 activation, Rubicon interacts with the CYBA subunit of the NAPDH oxidase complex, leading to a burst of reactive oxygen species and inflammatory cytokines (2).

Molekulargewicht: Predicted: 90, 101, 109 kDa

Observed: 91, 99 kDa

Gen-ID: 9711

NCBI Accession: XP_005269431

UniProt: Q92622

Pathways: Autophagie