GLS2 Antikörper (AA 300-350)

Produktnummer ABIN6991498

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch GLS2 in WB, ELISA, IHC (p) und IF. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für GLS2 Antikörper (AA 300-350) (ABIN6991498)

Target: GLS2 (Glutaminase 2 (Liver, Mitochondrial) (GLS2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GLS2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Produktdetails anti-GLS2 Antikörper

Bindungsspezifität: AA 300-350

Spezifität: Multiple isoforms of GLS2 are known to exist.

Aufreinigung: GLS2 Antibody is affinity chromatography purified via peptide column.

Immunogen: GLS2 antibody was raised against an 18 amino acid synthetic peptide near the center terminus of human GLS2. The immunogen is located within amino acids 300 - 350 of GLS2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: GLS2 antibody can be used for detection of GLS2 by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in rat samples, Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: GLS2 Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: GLS2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu GLS2

Target: GLS2 (Glutaminase 2 (Liver, Mitochondrial) (GLS2))

Andere Bezeichnung: GLS2

Hintergrund: GLS2 Antibody: Phosphate-activated glutaminase, also known as Glutaminase 2 (GLS2), was initially isolated from rat liver, although it has been shown to be expressed in other tissues. Like the functionally similar, larger kidney glutaminase, GLS2 catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Expression of GLS2 is increased by p53 under both stressed and nonstressed conditions, resulting in increased levels of glutamate and alpha-ketoglutarate, which in turn results in enhanced mitochondrial respiration and ATP generation. GLS2 also regulates antioxidant defense function in cells by increasing reduced glutathione levels and decreasing ROS-levels, suggesting that GLS2 acts as a mediator of p53's role in antioxidant defense in addition to its role in energy metabolism.

Molekulargewicht: Predicted: 36, 55, 62, 66 kDa

Observed: 55, 62 kDa

Gen-ID: 27165

NCBI Accession: NP_037399

UniProt: Q9UI32

Pathways: Dicarboxylic Acid Transport, Warburg Effekt