ATG13 Antikörper (C-Term)

Produktnummer ABIN6991353

Dieses Anti-ATG13-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von ATG13 in WB, ELISA, IHC (p) und IF. Geeignet für Human, Maus und Ratte.

Kurzübersicht für ATG13 Antikörper (C-Term) (ABIN6991353)

Target: ATG13 (Autophagy Related 13 (ATG13))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATG13 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Produktdetails anti-ATG13 Antikörper

Bindungsspezifität: AA 450-500, C-Term

Spezifität: Multiple isoforms of ATG13 are known to exist.

Aufreinigung: ATG13 Antibody is affinity chromatography purified via peptide column.

Immunogen: ATG13 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human ATG13. The immunogen is located within amino acids 450 - 500 of ATG13.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ATG13 antibody can be used for detection of ATG13 by Western blot at 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in rat samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: ATG13 Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: ATG13 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu ATG13

Target: ATG13 (Autophagy Related 13 (ATG13))

Andere Bezeichnung: ATG13

Hintergrund: ATG13 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein ATG1. ATG13 forms a complex with ULK1 and ULK2, the mammalian homologs of ATG1, and with FIP200. This complex is a target of TOR phosphorylation under normal conditions, inhibition of TOR by rapamycin or leucine deprivation leads to dephosphorylation of ATG13, ULK1 and ULK2, which then leads to autophagy. Knockdown of ATG13 inhibits autophagosome formation.

Molekulargewicht: Predicted: 44, 53, 57, 61 kDa

Observed: 49 kDa

Gen-ID: 9776

NCBI Accession: NP_001136145

UniProt: O75143

Pathways: PI3K-Akt Signalweg, Autophagie