RSPO1 Antikörper (N-Term)

Produktnummer ABIN6991124

Der Kaninchen Polyklonal Anti-RSPO1-Antikörper wurde für WB, ELISA, IHC (p) und IF validiert. Er ist geeignet, RSPO1 in Proben von Human und Maus zu detektieren.

Kurzübersicht für RSPO1 Antikörper (N-Term) (ABIN6991124)

Target: RSPO1 (R-Spondin 1 (RSPO1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RSPO1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Produktdetails anti-RSPO1 Antikörper

Bindungsspezifität: N-Term

Spezifität: At least three isoforms of RSPO1 are known to exist, this antibody will detect the largest and smallest isoforms. RSPO1 antibody will not cross-react with other RSPO family members.

Aufreinigung: RSPO1 Antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-RSPO1 antibody was raised against a peptide corresponding to 16 amino acids near the amino terminus of human RSPO1. The immunogen is located within the first 50 amino acids of RSPO1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 0.5-2 μ,g/mL, IHC: 2.5-5 μ,g/mL, IF: 5 μ,g/mL.

Antibody validated: Western Blot in human and mouse samples, Immunohistochemistry in human samples, Immunofluorescence in human samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: RSPO1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: RSPO1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu RSPO1

Target: RSPO1 (R-Spondin 1 (RSPO1))

Andere Bezeichnung: RSPO1

Hintergrund: RSPO1 Antibody: RSPO1 is a member of a family of secreted growth factors that can operate through the canonical Wnt signaling pathway by stabilizing the intracellular beta-catenin, thereby regulating functions mediated by beta-catenin such as cell fate decisions and embryonic patterning. RSPO1 was recently identified through linkage analysis to be involved in sex determination and mammalian ovarian development. RSPO1 is thought to regulate cellular responsiveness to Wnt ligands by modulating the cell-surface expression of the Wnt co-receptor LRP6 by interfering with the DKK/Kremen-mediated internalization of LRP6 through an interaction with Kremen, resulting in increased LRP6 cell-surface levels.

Molekulargewicht: Predicted: 29kD

Observed: 36 kD (Post-modification: 1 N-linked glycosylation) kDa

Gen-ID: 284654

NCBI Accession: NP_001033722

UniProt: Q2MKA7