PIGY Antikörper (AA 50-100)

Produktnummer ABIN6991033

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch PIGY in ELISA, WB, IHC (p) und IF. Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für PIGY Antikörper (AA 50-100) (ABIN6991033)

Target: PIGY (Phosphatidylinositol Glycan Anchor Biosynthesis, Class Y (PIGY))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PIGY Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Produktdetails anti-PIGY Antikörper

Bindungsspezifität: AA 50-100

Spezifität: This antibody only detects isoform 1 of PIG-Y.

Aufreinigung: PIG-Y Antibody is affinity chromatography purified via peptide column.

Immunogen: PIG-Y antibody was raised against a 16 amino acid synthetic peptide near the center of human PIG-Y. The immunogen is located within amino acids 50 - 100 of PIG-Y.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: PIG-Y antibody can be used for detection of PIG-Y by Western blot at 1 - 2 μ,g/mL. Despite its predicted molecular weight, PIG-Y often migrates at 28-30 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PIG-Y Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: PIG-Y antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu PIGY

Target: PIGY (Phosphatidylinositol Glycan Anchor Biosynthesis, Class Y (PIGY))

Andere Bezeichnung: PIG-Y

Hintergrund: PIG-Y Antibody: Glycosylphosphatidylinositol (GPI) lipid anchoring is an important post-translational modification of proteins that takes place in the endoplasmic reticulum. The synthesis of GPI is initiated by GPI-N-acetylglucosaminyltransferase (GPI-GnT), a complex of proteins including PIG-A, PIG-H, PIG-C, GPI1, and DPM2. PIG-Y, the mammalian homolog to yeast Eri1p, is also thought to be involved in the biosynthesis of GPI. The PIG-Y gene encodes two proteins, one of which arises from leaky scanning of the mRNA.

Molekulargewicht: Predicted: 8 kDa

Observed: 16 kDa

Gen-ID: 84992

NCBI Accession: NP_116295

UniProt: Q3MUY2

Pathways: Inositol Metabolic Process