ASK1 Antikörper (C-Term)

Produktnummer ABIN6990602

Der Kaninchen Polyklonal Anti-ASK1-Antikörper wurde für WB, ELISA, IF und ICC validiert. Er ist geeignet, ASK1 in Proben von Human und Maus zu detektieren.

Kurzübersicht für ASK1 Antikörper (C-Term) (ABIN6990602)

Target: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ASK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-ASK1 Antikörper

Bindungsspezifität: AA 1260-1310, C-Term

Aufreinigung: ASK1 Antibody is affinity chromatography purified via peptide column.

Immunogen: ASK1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ASK1. The immunogen is located within amino acids 1260 - 1310 of ASK1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ASK1 antibody can be used for the detection of ASK1 by Western blot at 0.5 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 2 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: ASK1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: ASK1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu ASK1

Target: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Andere Bezeichnung: ASK1

Hintergrund: ASK1 Antibody: Mitogen-activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5. ASK1 activated two different subgroups of MAPKK, MKK4 and MKK6, which in turn activated c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induced apoptotic cell death, and a catalytically inactive form of ASK1 inhibited TNF-α,-induced apoptosis. ASK1 is expressed in variety of tissues and cell lines.

Gen-ID: 4217

UniProt: Q99683

Pathways: MAPK Signalweg, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response