IRAK2 Antikörper (C-Term)

Produktnummer ABIN6990565

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch IRAK2 in WB, ELISA, ICC und IF. Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für IRAK2 Antikörper (C-Term) (ABIN6990565)

Target: IRAK2 (Interleukin-1 Receptor-Associated Kinase 2 (IRAK2))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IRAK2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Produktdetails anti-IRAK2 Antikörper

Bindungsspezifität: C-Term

Spezifität: At least four isoforms of IRAK2 are known to exist, this antibody will detect all four isoforms. Anti-IRAK2 has no cross response to IRAK.

Aufreinigung: IRAK2 Antibody is affinity chromatography purified via peptide column.

Immunogen: IRAK2 antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of mouse IRAK2a. The immunogen is located within the last 50 amino acids of IRAK2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IRAK2 antibody can be used for detection of IRAK2 by Western blot at 0.5 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 1 μ,g/mL. For immunofluorescence start at 10 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunocytochemistry in mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: IRAK2 Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: IRAK2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu IRAK2

Target: IRAK2 (Interleukin-1 Receptor-Associated Kinase 2 (IRAK2))

Andere Bezeichnung: IRAK2

Hintergrund: IRAK2 Antibody: The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-κ,B, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family has been identified and designated IRAK2. Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-κ,B activation. IRAKs also associate with Toll like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kB activation. Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. Unlike human IRAK2, murine IRAK2 exists as four alternately spliced isoforms (IRAK2a-d), with two isoforms (IRAK2c and d) acting in an inhibitory fashion. IRAK2 is expressed in a variety of tissues.

Molekulargewicht: Predicted: 68 kDa

Observed: 65 kDa

Gen-ID: 108960

UniProt: Q8CFA1

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades