Caspase 12 Antikörper (AA 170-220)

Produktnummer ABIN6990234

Dieses Anti-Caspase 12-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Caspase 12 in WB, ELISA, IF und IHC (p). Geeignet für Human, Maus und Ratte.

Kurzübersicht für Caspase 12 Antikörper (AA 170-220) (ABIN6990234)

Target: Caspase 12 (CASP12) (Caspase 12 (Gene/pseudogene) (CASP12))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Caspase 12 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Caspase 12 Antikörper

Bindungsspezifität: AA 170-220

Spezifität: Caspase-12 (large) antibody is predicted to have no cross reactivity to other members in the caspase family.

Aufreinigung: Caspase-12 (Large) Antibody is affinity chromatography purified via peptide column.

Immunogen: Caspase-12 antibody was raised against a synthetic peptide corresponding to 16 amino acids within the large cleavage product of murine caspase-12. The immunogen is located within amino acids 170 - 220 of Caspase-12.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Caspase-12 (large) antibody can be used for the detection of both the propeptide and cleaved forms of caspase-12 by Western blot at 0.25 to 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2 μ,g/mL. For immunofluorescence start at 10 μ,g/mL.

Antibody validated: Western Blot in human, mouse, and rat samples, Immunohistochemistry in human, mouse, and rat samples and Immunofluorescence in human, mouse, and rat samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Caspase-12 Antibody is supplied in PBS containing 0.02 % sodium azide. (Large)

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Caspase-12 (Large) antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu Caspase 12

Target: Caspase 12 (CASP12) (Caspase 12 (Gene/pseudogene) (CASP12))

Andere Bezeichnung: Caspase-12

Hintergrund: Caspase-12 Antibody: (Large)Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the main, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved. Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer's disease including gamma-secretase presenilin and beta-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-beta. Caspase-12 is ubiquitously expressed in mouse tissues.

Molekulargewicht: Predicted: 46 kDa

Observed: 46 kDa

Gen-ID: 12364

UniProt: O08736

Pathways: Apoptose, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response