Rekombinanter Cyclin E2 Antikörper (C-Term)

Produktnummer ABIN6989714

Der Kaninchen Monoklonal anti-Cyclin E2 Antikörper wird verwendet zum Nachweis von Cyclin E2 in Proben von Human. Er wurde validiert für WB, FACS, IHC (p), IF (cc), IHC (fro) und IF (p).

Kurzübersicht für Rekombinanter Cyclin E2 Antikörper (C-Term) (ABIN6989714)

Target: Cyclin E2 (CCNE2)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Cyclin E2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Produktdetails anti-Cyclin E2 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Cyclin E2 Recombinant Antibody

Kreuzreaktivität: Human

Aufreinigung: Purified by Protein A.

Immunogen: Recombinant human Cyclin E2 protein, around C-terminal 100aa.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB(WB(1:500-2000)), FCM(FCM(1:50-100)), IHC-P(IHC-P(1:50-200)), IHC-F(IHC-F(1:50-200)), IF(IHC-P)(IF(IHC-P)(1:50-200)), IF(ICC)(IF(ICC)(1:50-200))

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.01M TBS ( pH 7.4), 1 % BSA, 0.02 % Proclin 300, and 50 % Glycerol

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu Cyclin E2

Target: Cyclin E2 (CCNE2)

Andere Bezeichnung: Cyclin E2

Hintergrund:

Synonyms: Cyclin E2 phospho T392, p-Cyclin E2 phospho T392,CCN E2, CCNE 2, CCNE2, CCNE2 protein, CYC E2, CYCE 2, CYCE2, CyclinE2, G1/S-specic cyclin E2, G1/S-specic cyclin E2.

Background: The human Cyclin E2 gene encodes a 404 amino acid protein that is most closely related to Cyclin E. Cyclin E2 mRNA levels peaks at the G1 / S transition. Cyclin E2 associates with Cdk2 in a functional kinase complex that is inhibited by both p27 (Kip1) and p21 (Cip1). Cyclin E2 / Cdk2 phosphorylates histone H1 in vitro. G1 cyclin E controls the initiation of DNA synthesis by activating CDK2. Abnormally high levels of cyclin E expression have frequently been observed in human cancers. Unlike Cyclin E1, which is expressed in great majority of proliferating normal and neoplastically transformed cells, Cyclin E2 levels are low to undetectable in non transformed cells and increase significantly in neoplasm derived cells.

Gen-ID: 9134

UniProt: O96020

Pathways: Zellzyklus, Mitotic G1-G1/S Phases