CHEK2 Antikörper (pSer33, pSer35) (AbBy Fluor® 680)

Produktnummer ABIN6980774

Der Kaninchen Polyklonal Anti-CHEK2-Antikörper wurde für WB, IF (cc) und IF (p) validiert. Er ist geeignet, CHEK2 in Proben von Human zu detektieren.

Kurzübersicht für CHEK2 Antikörper (pSer33, pSer35) (AbBy Fluor® 680) (ABIN6980774)

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CHEK2 Antikörper ist konjugiert mit AbBy Fluor® 680

Applikation: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Produktdetails anti-CHEK2 Antikörper (AbBy Fluor® 680)

Bindungsspezifität: pSer33, pSer35

Kreuzreaktivität: Human

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated Synthesised phosphopeptide derived from human CHK2 isoform c around the phosphorylation site of Ser33 + Ser35

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu CHEK2

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Andere Bezeichnung: CHK2 (

Hintergrund:

Synonyms: Chk2 (phospho S33 + S35), p-Chk2 (phospho S33 + S35), bA444G7, CHK2 checkpoint homolog, CHK2_HUMAN, Serine/threonine-protein kinase Chk2, CDS 1, CDS1, Checkpoint kinase 2, Checkpoint like protein CHK2, Chek 2, Chek2, Chk 2, CHK2 checkpoint homolog (S. pombe), CHK2 checkpoint homolog, HuCds 1, HuCds1, LFS 2, LFS2, PP1425, RAD 53, RAD53, Rad53 homolog, Serine/threonine protein kinase Chk2.

Background: In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Gen-ID: 11200

UniProt: O96017

Pathways: p53 Signalweg, Apoptose, Zellzyklus