H2AFZ Antikörper (C-Term)

Produktnummer ABIN6971773

Produktdetails anti-H2AFZ Antikörper

Target: H2AFZ (H2A Histone Family, Member Z (H2AFZ))

Bindungsspezifität: C-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser H2AFZ Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq), Dot Blot (DB)

Produktmerkmale: The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Histone H2A.Z (H2AZ, H2AFZ) is a histone H2A variant, a protein similar to canonical H2A but with different molecular identity and unique functions. H2A.Z is highly conserved during evolution. It plays an important role in basic cellular mechanisms such as gene activation, chromosome segregation, heterochromatic silencing and progression through the cell cycle. H2A.Z is acetylated at multiple lysine residues in its amino terminus, which may enable H2A.Z to function as an insulator of chromatin domains. Histone H2A.Z antibody (pAb) (H2AZ) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Dot blot, Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.

Aufreinigung: Protein A Chromatography

Immunogen: This Histone H2A.Z antibody was raised against a peptide derived from the C-terminus of human histone H2A.Z.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Details zu H2AFZ

Target: H2AFZ (H2A Histone Family, Member Z (H2AFZ))

Andere Bezeichnung: Histone H2A.Z (H2AFZ Produkte)

Synonyme: H2A.Z-1 antikoerper, H2A.z antikoerper, H2A/z antikoerper, H2AZ antikoerper, H2A.Z antikoerper, H2a.z-1 antikoerper, H2AFV antikoerper, H2Az antikoerper, XH2AZ antikoerper, h2a.zl1 antikoerper, h2a.zl2 antikoerper, H2A histone family member Z antikoerper, H2A histone family, member Z antikoerper, H2A histone family member Z L homeolog antikoerper, histone H2A.Z antikoerper, H2AFZ antikoerper, H2afz antikoerper, h2afz.L antikoerper

Molekulargewicht: 14 kDa

NCBI Accession: NP_002097

Pathways: Telomere Maintenance