IL-2 Antikörper (PromoFluor-488 Premium)
Kurzübersicht für IL-2 Antikörper (PromoFluor-488 Premium) (ABIN6963755)
Target
Alle IL-2 (IL2) Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Spezifität
- Native and recombinant IL-2
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Kreuzreaktivität (Details)
- The monoclonal antibody cross-reacts with IL-2 from sheep. For dection of IL-2 in sheep, unconjugated MT3B3 with second step detection is recommended.
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Aufreinigung
- Purified from in vitro cultures by protein G affinity chromatography.
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Immunogen
- Recombinant bovine IL-2
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Isotyp
- IgG1
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anti-Interleukin 2 (IL2) (AA 21-153) antibody
IL2 Reaktivität: Human WB, IHC, IP, ICC Wirt: Kaninchen Polyclonal unconjugated
anti-Interleukin 2 (IL2) (AA 21-153) antibodyIL2 Reaktivität: Human WB, IHC, IP, ICC Wirt: Maus Monoclonal C3 unconjugated
anti-Interleukin 2 (IL2) (AA 10-169) antibodyIL2 Reaktivität: Maus WB, IHC, IP, ICC Wirt: Kaninchen Polyclonal unconjugated
anti-Interleukin 2 (IL2) (AA 21-153) antibodyIL2 Reaktivität: Human WB Wirt: Maus Monoclonal C5 unconjugated
anti-Interleukin 2 (IL2) antibodyIL2 Reaktivität: Human WB, ELISA, FACS Wirt: Kaninchen Monoclonal unconjugated
anti-Interleukin 2 (IL2) antibodyIL2 Reaktivität: Human WB, IP, ELISA, ICC, FACS Wirt: Maus Monoclonal 35C3 unconjugated
anti-Interleukin 2 (IL2) antibody (APC)IL2 Reaktivität: Human ICFC Wirt: Maus Monoclonal 35C3 APC
anti-Interleukin 2 (IL2) antibody (PE)IL2 Reaktivität: Human FACS Wirt: Maus Monoclonal 35C3 PE
anti-Interleukin 2 (IL2) (AA 21-155) antibodyIL2 Reaktivität: Ratte WB, IHC, IP, ICC Wirt: Kaninchen Polyclonal unconjugated
anti-Interleukin 2 (IL2) (AA 10-169) antibodyIL2 Reaktivität: Maus WB, IHC, IP, ICC Wirt: Maus Monoclonal C12 unconjugated
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Applikationshinweise
- Detection of IL-2 producing cells by flow cytometry. 5 μL is recommended for staining of 1 million cells in a total volume of 50 μL. Detection of IL-2 by flow cytometry in viable bovine PBMC. Cells were stimulated for 5 hours in the presence of PMA/ionomycin and Brefeldin A. Cells were then fixed and permeabilized using 4 % paraformaldehyde and saponin, and subsequently stained with anti- bovine IL-2 mAb MT3B3-PF488P (solid line). Matched isotype control antibody MT128. The histogram derives from gated events of typical lymphocyte characteristics in forward and side light scatter. Flow cytometry was performed on a BD FACSVerse system.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Buffer
- in PBS with 0.2 % BSA and 0.09 % sodium azide
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
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Haltbarkeit
- 18 months
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- IL-2 (IL2) (Interleukin 2 (IL2))
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Andere Bezeichnung
- IL2
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Gen-ID
- 280822
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Pathways
- JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
Target
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