CD3 Antikörper (violetFluor™ 500)

Produktnummer ABIN7278611

Dieses Anti-CD3-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CD3 in FACS. Geeignet für Human.

Kurzübersicht für CD3 Antikörper (violetFluor™ 500) (ABIN7278611)

Target: CD3

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD3 Antikörper ist konjugiert mit violetFluor™ 500

Applikation: Flow Cytometry (FACS)

Klon: UCHT1

Produktdetails anti-CD3 Antikörper (violetFluor™ 500)

Kreuzreaktivität: Schimpanse

Aufreinigung: This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: This antibody preparation has been pre-titrated and quality-tested for flow cytometry using an appropriate cell type. The antibody has been diluted for use at 5 μLper test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 uL. The number of cells within a sample should be determined empirically, but typically ranges between 1x10e5 to 1x10e8 cells. violetFluor™ 500 dye is excited by the violet (405 nm) laser and has a peak emission of 500 nm. The recommended band pass filter for this dye is 525/20. violetFluor™ 500 can be used as an alternative for BD Horizon™ V500 or Pacific Orange®.

Kommentare:

5 uL (0.5 ug)/test

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide, 0.1 % gelatin, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: 2-8°C protected from light

Haltbarkeit: 12 months

Details zu CD3

Target: CD3

Andere Bezeichnung: CD3

Hintergrund: The UCHT1 antibody is specific for human CD3e, also known as CD3 epsilon, a 20 kDa subunit of the T cell receptor complex. Together with CD3 gamma, CD3 delta and CD3 zeta, CD3 epsilon assembles to form the CD3 complex that associates with the T cell receptor (TCR). Along with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. These interactions promote T cell receptor signaling (T cell activation), inducing cell proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte development and on all mature T cells.The UCHT1 antibody is a widely used phenotypic marker for human T cells. In addition, binding/cross-linking of UCHT1 antibody to CD3e can induce cell activation (use format suitable for functional assays).

Gen-ID: 915, 916, 917

UniProt: P20963, A8MVP6, P07766, P09693

Pathways: T-Zell Rezeptor Signalweg, Ubiquitin Proteasome Pathway