CD3 Antikörper
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- Target Alle CD3 Antikörper anzeigen
- CD3
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser CD3 Antikörper ist unkonjugiert
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Applikation
- Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Immunofluorescence (IF), Western Blotting (WB)
- Kreuzreaktivität
- Schimpanse
- Aufreinigung
- This monoclonal antibody preparation was purified from tissue culture supernatant via affinity chromatography. For In Vivo Ready™ (IVR) products, each preparation is also evaluated for endotoxin levels using the LAL assay. It is recommended to store the product undiluted at 4°C. Do not freeze.
- Endotoxin-Niveau
- Low endotoxin: < 0.01 EU/ug
- Klon
- UCHT1
- Isotyp
- IgG1 kappa
- Top Product
- Discover our top product CD3 Primärantikörper
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- Applikationshinweise
- This purified format is guaranteed to be >90 % pure as determined by SDS-PAGE analysis.
- Kommentare
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2 mg/mL
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Buffer
- 10 mM NaH2PO4, 150 mM NaCl, pH 7.2
- Konservierungsmittel
- Azide free
- Lagerung
- 4 °C
- Informationen zur Lagerung
- 2-8°C
- Haltbarkeit
- 12 months
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- Target
- CD3
- Andere Bezeichnung
- CD3 (CD3 Produkte)
- Synonyme
- CD3 antikoerper, AI504783 antikoerper, CD3epsilon antikoerper, T3e antikoerper, cd3-gamma antikoerper, cd3g antikoerper, cd3g-a antikoerper, cd3g/d antikoerper, cd3gd antikoerper, t3g antikoerper, CD3e molecule antikoerper, CD3d molecule antikoerper, CD3 antigen, epsilon polypeptide antikoerper, CD3g molecule S homeolog antikoerper, CD3E antikoerper, CD3D antikoerper, Cd3e antikoerper, cd3g.S antikoerper
- Hintergrund
- The UCHT1 antibody is specific for human CD3e, also known as CD3 epsilon, a 20 kDa subunit of the T cell receptor complex, along with CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor - CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. These interactions promote T cell receptor signaling (T cell ac- tivation), inducing cell proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells.The UCHT1 antibody is a widely used phenotypic marker for human T cells. In addition, binding/cross-linking of UCHT1 antibody to CD3e can induce cell activation. A recent publication of the crystal structure of a CD3e- antibody complex provides insight as to the action of commonly used agonist antibodies, as well as specific epitope-binding data for the human CD3 antibodies UCHT1 and OKT3 (Fernandes, R.A. et al. 2012. J. Biol. Chem. 287: 13324-13335).UCHT1 antibody reacts with both surface-expressed and intracellular CD3e protein, in contrast to an alternative human CD3 clone, HIT3a, which will stain only the extracellular (membrane-expressed) CD3e protein. Also, the UCHT1 antibody is reported to be cross-reactive with chimpanzee and has been used for phenotypic analysis of expression by flow cytometry - however the antibody is reported to be unsuitable for induc- tion of T cell activation in this species (Bibollet-Ruche et al. 2009. J. Virol. 82: 10271-10278).
- Gen-ID
- 915, 916, 917
- UniProt
- P20963, A8MVP6, P07766, P09693
- Pathways
- T-Zell Rezeptor Signalweg, Ubiquitin Proteasome Pathway
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