LRRC32 Antikörper (APC)

Produktnummer ABIN7278040

Dieses Anti-LRRC32-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von LRRC32 in FACS. Geeignet für Human.

Kurzübersicht für LRRC32 Antikörper (APC) (ABIN7278040)

Target: LRRC32 (Leucine Rich Repeat Containing 32 (LRRC32))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser LRRC32 Antikörper ist konjugiert mit APC

Applikation: Flow Cytometry (FACS)

Klon: GARP5

Produktdetails anti-LRRC32 Antikörper (APC)

Aufreinigung: This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: This antibody preparation has been pre-titrated and quality-tested for flow cytometry using an appropriate cell type. The antibody has been diluted for use at 5 μLper test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 uL. The number of cells within a sample should be determined empirically, but typically ranges between 1x10e5 to 1x10e8 cells.

Kommentare:

5 uL (0.5 ug)/test

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide, 0.1 % gelatin, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: 2-8°C protected from light

Haltbarkeit: 12 months

Details zu LRRC32

Target: LRRC32 (Leucine Rich Repeat Containing 32 (LRRC32))

Andere Bezeichnung: GARP

Hintergrund: The GARP5 antibody reacts with human GARP, also known as LRRC32 or Garpin, an 80 kDa transmembrane protein which acts as a receptor for the latent form of TGF-beta 1 (pro-TGF-beta), preventing its secretion. Specifically, GARP is reported to associate with the pro-domain of TGF-beta 1, known as latency-associated peptide (LAP), which must be cleaved to release the biologically active cyto- kine. The putative role of GARP in sequestering the TGF-beta is important for regulating its activity, as TGF-beta signaling is involved in development and function of Th17, Treg and many other immune cell types. GARP expression has been shown on platelets and at high levels on Foxp3+ Treg cells, where it is proposed to be a phenotypic identifier for activated human Treg cells. The GARP5 antibody may be used for analysis of human GARP by flow cytometry, immunoprecipitation and Western blotting.

Gen-ID: 2615

Pathways: Activated T Cell Proliferation