Keratin 5/8 Antikörper
Kurzübersicht für Keratin 5/8 Antikörper (ABIN6941336)
Target
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Spezifität
- It reacts with keratin 5 (58 kDa) and keratin 8 (52.5 kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.
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Keine Kreuzreaktivität
- Huhn, Xenopus laevis
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Aufreinigung
- Purified by Protein A/G
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Immunogen
- Cytoskeletal preparation from HeLa cells
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Isotyp
- IgG1 kappa
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Applikationshinweise
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Positive Control: HepG2 or A431 cells. Skin or Squamous cell carcinoma.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1.0 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 min at RT)(For staining of formalin-fixed tissues, digest sections with trypsin at 1 mg/mL PBS, 15 min at RT)Optimal dilution for a specific application should be determined.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Konzentration
- 200 μg/mL
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Buffer
- 0.5 mL at 200 µg/mL of antibody Purified from Bioreactor Concentrate by Protein A/G chromatography. Supplied in 10 mM PBS, pH 7.4 with 0.05 % BSA and 0.05 % sodium azide. Contact us if you require this antibody in a different format.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C
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Informationen zur Lagerung
- Store at 2 to 8°C. Antibody is stable for 24 months.
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Haltbarkeit
- 24 months
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