CD74 Antikörper
Kurzübersicht für CD74 Antikörper (ABIN6941256)
Target
Alle CD74 Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Spezifität
- It recognizes a protein of ~35 kDa, identified as CD74. It is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).
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Aufreinigung
- Purified by Protein A/G
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Immunogen
- Recombinant full-length human CD74 protein
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Isotyp
- IgG1 kappa
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Applikationshinweise
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Positive Control: Daudi or Raji cells. Tonsil or lymph node.
Known Application: Flow Cytometry (1-2 μg/million cells) (Methanol Fixed), ,Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Konzentration
- 200 μg/mL
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Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C,-80 °C
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Informationen zur Lagerung
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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Haltbarkeit
- 24 months
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- CD74 (HLA-DR-gamma (CD74))
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Andere Bezeichnung
- CD74
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Molekulargewicht
- 33-41kDa
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Gen-ID
- 972
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Pathways
- Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints
Target
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