Rekombinanter CD44 Antikörper

Produktnummer ABIN6941200

Der Maus Monoklonal Anti-CD44-Antikörper wurde für FACS, WB, IHC, IF, Func und StM validiert. Er ist geeignet, CD44 in Proben von Human, Pavian und Green Monkey zu detektieren.

Kurzübersicht für Rekombinanter CD44 Antikörper (ABIN6941200)

Target: CD44

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Pavian, Green Monkey

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD44 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Functional Studies (Func), Staining Methods (StM)

Klon: RHCAM-918

Produktdetails anti-CD44 Antikörper

Spezifität: Recognizes a cell surface glycoprotein of 80-95 kDa (CD44) on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V). Its epitope is resistant to digestion by trypsin and chymotrypsin. This MAb selectively interferes with lymphocyte binding to lymph node, mucosal and synovial endothelium. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95 kDa or hematopoietic variant (CD44s). Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant full-length human CD44 protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: HeLa cells (FACS). HeLa cell lysate (WB). Paracortex in tonsil or lymph node (IHC).

Known Application: Functional Studies (Order Ab without BSA & Azide), Western Blot (1-2 μg/mL), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu CD44

Target: CD44

Andere Bezeichnung: CD44

Molekulargewicht: 80-95kDa

Gen-ID: 960

UniProt: P16070

Pathways: Glycosaminoglycan Metabolic Process, Autophagie, Negative Regulation of intrinsic apoptotic Signaling