CD22 Antikörper (AA 52-178)

Produktnummer ABIN6941120

Dieses Maus Monoklonal-Antikörper erkennt spezifisch CD22 in FACS, WB, ELISA, IHC, IF, Coat und StM. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für CD22 Antikörper (AA 52-178) (ABIN6941120)

Target: CD22 (CD22 Molecule (CD22))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD22 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Coating (Coat), Staining Methods (StM)

Klon: BLCAM-1795

Produktdetails anti-CD22 Antikörper

Bindungsspezifität: AA 52-178

Spezifität: Recognizes a protein of 130-140 kDa, identified as CD22 (also known as BL-CAM). CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation.

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant fragment (around aa52-178) of human BL-CAM/CD22 protein (exact sequence is proprietary)

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Raji, Ramos, MOLT4 and human peripheral blood lymphocytes. Tonsil, Lymph Node or Spleen.

Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA),Flow Cytometry (1-2 μg/million cells),Western Blot (1-2 μg/mL),Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu CD22

Target: CD22 (CD22 Molecule (CD22))

Andere Bezeichnung: CD22

Molekulargewicht: 130-140kDa

Gen-ID: 933

UniProt: P20273