Cyclin B2 Antikörper

Produktnummer ABIN6941010

Produktdetails anti-Cyclin B2 Antikörper

Target: Cyclin B2 (CCNB2)

Reaktivität: Human, Maus, Ratte, Xenopus laevis

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cyclin B2 Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Coating (Coat)

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant full-length Xenopus laevis Cyclin B2 protein

Klon: X29-2

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: HeLa cells. Human cervical tissue (IHC).

Known Application: ELISA (For coating, order antibody without BSA), Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Cyclin B2

Target: Cyclin B2 (CCNB2)

Andere Bezeichnung: CCNB2 (CCNB2 Produkte)

Synonyme: HsT17299 antikoerper, CycB2 antikoerper, sb:cb303 antikoerper, cycB2 antikoerper, LOC100136691 antikoerper, C-B2 antikoerper, cyclinb2 antikoerper, hst17299 antikoerper, LOC100282653 antikoerper, cyclin B2 antikoerper, cyclin B2 S homeolog antikoerper, CCNB2 antikoerper, Ccnb2 antikoerper, ccnb2 antikoerper, LOC100136691 antikoerper, LOC100282653 antikoerper, ccnb2.S antikoerper

Hintergrund: In eukaryotic cells, mitosis is initiated following the activation of a protein kinase known variously as maturation-promoting factor, M phase specific histone kinase or M-phase kinase. This protein kinase is composed of a catalytic subunit (Cdc2), a regulatory subunit (cyclin B) and a low molecular weight subunit (p13-Suc1). The Cdc/cyclin enzyme is subject to multiple levels of control, of which the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/ cyclin B enzyme, and tyrosine dephosphorylation, occurring at the onset of mitosis, directly activates the pre-MPF complex. Evidence has established that B type cyclins not only act on M phase regulatory subunits of the Cdc2 protein kinase, but also activate the Cdc25A and Cdc25B endogenous tyrosine phosphatase, of which Cdc2 is the physiological substrate. The two B type cyclins, cyclin B1 and cyclin B2, have been shown to have distinct tissue distributions.

Molekulargewicht: 51kDa

Gen-ID: 9133

UniProt: O95067

Pathways: Zellzyklus, M Phase