CAPNL1 Antikörper

Produktnummer ABIN6940954

Der Maus Monoklonal Anti-CAPNL1-Antikörper wurde für IHC, ELISA, Coat und StM validiert. Er ist geeignet, CAPNL1 in Proben von Human zu detektieren.

Kurzübersicht für CAPNL1 Antikörper (ABIN6940954)

Target: CAPNL1 (Calpain 1, Large Subunit (CAPNL1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CAPNL1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), ELISA, Coating (Coat), Staining Methods (StM)

Klon: CAPN1-1530

Produktdetails anti-CAPNL1 Antikörper

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant full-length human CAPN1 protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: HeLa cells. Skin or Tonsil.

Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu CAPNL1

Target: CAPNL1 (Calpain 1, Large Subunit (CAPNL1))

Andere Bezeichnung: CAPN1

Hintergrund: Calpain 1 is an intracellular calcium-dependent protease that cleaves cytoskeletal and sub-membranous proteins. Calpains are non-lysosomal, calcium-activated intracellular cysteine proteases. Calpains mediate specific Ca2+- dependent processes including cell fusion, mitosis and meiosis. Calpains are heterodimers of a small regulatory subunit and one of three large catalytic subunits, designated Calpain 1, Calpain 2 and Calpain p94. Calpastatin regulates Calpain by inhibiting both the proteolytic activity of Calpain and its binding to membranes. Calpastatin exists in two types, tissue type and erythrocyte type, resulting from both alternative splicing and proteolytic processing. Calpain 1 co-localizes with human leukocyte antigen-DR (HLA-DR) on activated microglia in the aging brain. Calpain influences the process of spermatogenesis and the events preceding fertilization, such as the acrosome reaction.

Molekulargewicht: 80kDa

Gen-ID: 823

UniProt: P07384

Pathways: Apoptose