CA8 Antikörper

Produktnummer ABIN6940916

Der Maus Monoklonal anti-CA8 Antikörper wird verwendet zum Nachweis von CA8 in Proben von Human. Er wurde validiert für WB und IHC.

Kurzübersicht für CA8 Antikörper (ABIN6940916)

Target: CA8 (Carbonic Anhydrase VIII (CA8))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CA8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: CPTC-CA8-2

Produktdetails anti-CA8 Antikörper

Verwendungszweck: Carbonic Anhydrase VIII Antibody

Spezifität: The protein encoded by this gene was initially named CA-related protein because of sequence similarity to other known carbonic anhydrase genes. However, the gene product lacks carbonic anhydrase activity (i.e., the reversible hydration of carbon dioxide). The gene product continues to carry a carbonic anhydrase designation based on clear sequence identity to other members of the carbonic anhydrase gene family. The absence of CA8 gene transcription in the cerebellum of the lurcher mutant in mice with a neurologic defect suggests an important role for this acatalytic form. Mutations in CA8 gene causes neuropathology, such as ataxia, mild mental retardation and the predisposition to quadrupedal gait. It is also associated with the development of colorectal and lung cancers. Additionally, it is upregulated in various cancers.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human CA8 protein

Isotyp: IgG2a, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Human cerebellum. Selective expression in purkinje cells. HEK-293 and K-562 cell lines.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu CA8

Target: CA8 (Carbonic Anhydrase VIII (CA8))

Andere Bezeichnung: CA8

Hintergrund:

CA-VIII, CALS, CARP, MGC120502, MGC99509

Human cerebellum. Selective expression in Purkinje cells. HEK293 or K-562 cell lines.

Molekulargewicht: 32.97kDa

Gen-ID: 767, 654388

UniProt: P35219