Topoisomerase II alpha Antikörper (AA 1352-1493)

Produktnummer ABIN6940773

Dieser Maus Monoklonal Antikörper erkennt spezifisch Topoisomerase II alpha in WB, IHC, IF und StM. Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für Topoisomerase II alpha Antikörper (AA 1352-1493) (ABIN6940773)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Topoisomerase II alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Staining Methods (StM)

Klon: TOP2A-1361

Produktdetails anti-Topoisomerase II alpha Antikörper

Bindungsspezifität: AA 1352-1493

Spezifität: It recognizes a 170 kDa protein, which is identified as topoisomerase II is also implicated in drug resistance of tumor cells and has been shown to be over-expressed in many human cancers. Decreased expression of Topo IIa is the predominant mechanism of resistance to several chemotherapeutic agents.

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant human Topoisomerase II alpha fragment (aa1352-1493)

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: HeLa cells. Tonsil.

Known Application: Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Andere Bezeichnung: TOP2A

Molekulargewicht: 170kDa

Gen-ID: 7153

UniProt: P11388

Pathways: Zellzyklus, Mitotic G1-G1/S Phases