Rekombinanter Retinoblastoma 1 Antikörper

Produktnummer ABIN6940481

Produktdetails anti-Retinoblastoma 1 Antikörper

Target: Retinoblastoma 1 (RB1)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Retinoblastoma 1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Staining Methods (StM)

Spezifität: Recognizes a 105 kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. Its epitope is localized between aa 703-772. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant human full-length Rb1 protein

Klon: RB1-2313R

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Positive Control: HeLa, A431 or BT-20 cells. Breast or Colon carcinoma.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Retinoblastoma 1

Target: Retinoblastoma 1 (RB1)

Andere Bezeichnung: RB1 (RB1 Produkte)

Synonyme: OSRC antikoerper, RB antikoerper, p105-Rb antikoerper, pRb antikoerper, pp110 antikoerper, Rb antikoerper, Rb-1 antikoerper, P105-RB antikoerper, PP110 antikoerper, RB11 antikoerper, zgc:154147 antikoerper, RB transcriptional corepressor 1 antikoerper, RB transcriptional corepressor like 1 antikoerper, retinoblastoma 1 antikoerper, RB1 antikoerper, RBL1 antikoerper, Rb1 antikoerper, rb1 antikoerper

Molekulargewicht: 105kDa

Gen-ID: 5925

UniProt: P06400

Pathways: Zellzyklus, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagie