RAD51 Antikörper (AA 1-134)

Produktnummer ABIN6940477

Der Maus Monoklonal anti-RAD51 Antikörper wird verwendet zum Nachweis von RAD51 in Proben von Human. Er wurde validiert für WB und ELISA.

Kurzübersicht für RAD51 Antikörper (AA 1-134) (ABIN6940477)

Target: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser RAD51 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Klon: RAD51-2753

Produktdetails anti-RAD51 Antikörper

Bindungsspezifität: AA 1-134

Verwendungszweck: RAD51 (Prognostic and Response to Chemotherapy Marker) Antibody

Spezifität: RAD51 is one of the key factors of DNA repair by homologous recombination and has been shown to have anti-apoptotic activity in tumor cells. RAD51 protein interacts with a variety of tumor suppressor proteins including p53, BRCA1 and BRCA2. Elevated expression of RAD51 enhances radio-resistance of human tumor cells. Overexpression of RAD51 protein in tumor cells renders them resistant against cytotoxic drugs like Cisplatin. RAD51 interacts with BRCA1 and BRCA2 to influence subcellular localization and cellular response to DNA damage. BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis from deregulation of RAD51. High-level expression of RAD51 has been observed in a variety of human malignancies.RAD51 overexpression correlates with histological grading of the tumor in invasive ductal mammary carcinoma.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment of human RAD51 protein (around aa 1-134) (exact sequence is proprietary)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Positive Control: HeLa or MCF-7 cells. Colon or breast Carcinoma.

Known Application: ELISA (For coating, order Ab without BSA),Western Blot (1-2 μg/mL)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu RAD51

Target: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Andere Bezeichnung: RAD51

Hintergrund:

RCA1/BRCA2 containing complex, subunit 5, BRCC5, DNA repair protein RAD51, FANCR, HsT16930, MRMV2, RAD51, RAD51 recombinase, RAD51A, RECA, RecA like protein, Recombination protein A

HeLa or MCF-7 cells. Colon or breast carcinoma.

Molekulargewicht: 37kDa

Gen-ID: 5888, 631709

UniProt: Q06609

Pathways: DNA Reparatur